Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Dual function of mitochondrial Nm23-H4 protein in phosphotransfer and intermembrane lipid transfer: a cardiolipin-dependent switch.

Schlattner-U; Tokarska-Schlattner-M; Ramirez-S; Tyurina-YY; Amoscato-AA; Mohammadyani-D; Huang-Z; Jiang-J; Yanamala-N; Seffouh-A; Boissan-M; Epand-RF; Epand-RM; Klein-Seetharaman-J; Lacombe-ML; Kagan-VE
J Biol Chem 2013 Jan; 288(1):111-121
The nucleoside diphosphate kinase Nm23-H4/NDPK-D forms symmetrical hexameric complexes in the mitochondrial intermembrane space with phosphotransfer activity using mitochondrial ATP to regenerate nucleoside triphosphates. We demonstrate the complex formation between Nm23-H4 and mitochondrial GTPase OPA1 in rat liver, suggesting its involvement in local and direct GTP delivery. Similar to OPA1, Nm23-H4 is further known to strongly bind in vitro to anionic phospholipids, mainly cardiolipin, and in vivo to the inner mitochondrial membrane. We show here that such protein-lipid complexes inhibit nucleoside diphosphate kinase activity but are necessary for another function of Nm23-H4, selective intermembrane lipid transfer. Mitochondrial lipid distribution was analyzed by liquid chromatography-mass spectrometry using HeLa cells expressing either wild-type Nm23-H4 or a membrane binding-deficient mutant at a site predicted based on molecular modeling to be crucial for cardiolipin binding and transfer mechanism. We found that wild type, but not the mutant enzyme, selectively increased the content of cardiolipin in the outer mitochondrial membrane, but the distribution of other more abundant phospholipids (e.g. phosphatidylcholine) remained unchanged. HeLa cells expressing the wild-type enzyme showed increased accumulation of Bax in mitochondria and were sensitized to rotenone-induced apoptosis as revealed by stimulated release of cytochrome c into the cytosol, elevated caspase 3/7 activity, and increased annexin V binding. Based on these data and molecular modeling, we propose that Nm23-H4 acts as a lipid-dependent mitochondrial switch with dual function in phosphotransfer serving local GTP supply and cardiolipin transfer for apoptotic signaling and putative other functions.
Animals; Animal-studies; Laboratory-animals; Lipids; Nucleosides; Cell-metabolism; Metabolism; Protein-biochemistry; Protein-chemistry; Proteins; Phospholipids; Liver-microsomal-metabolism; Liver-cells; Chemical-binding; Cell-damage; Cellular-structures; Physiological-chemistry; Biochemical-analysis; Liquid-chromatography; Mass-spectrometry; Enzyme-activity; Enzymes; Cytochemistry
Uwe Schlattner, Laboratory of Fundamental and Applied Bioenergetics, University Joseph Fourier, Inserm U1055, BP 53, F-38041 Grenoble Cedex 9, France
Publication Date
Document Type
Journal Article
Email Address
Funding Type
Fiscal Year
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-008282; B20130321
Issue of Publication
Source Name
Journal of Biological Chemistry
Performing Organization
University of Pittsburgh at Pittsburgh