Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

CYP2E1 mRNA expression, genetic polymorphisms in peripheral blood lymphocytes and liver abnormalities in Chinese VCM-exposed workers.

Authors
Wang-A-H; Zhu-S-M; Qiu-Y-L; Zhu-R; Qu-Y-B; Li-Y-L; Brandt-Rauf-P; Xia-Z-L
Source
Int J Occup Med Environ Health 2008 Jun; 21(2):141-146
NIOSHTIC No.
20042203
Abstract
Objective: To study the relationship between expression of cytochrome P4502E1 (CYP2E1) in human lymphocytes, variant CYP2E1 genotype, exposure to vinyl chloride monomer (VCM), and liver abnormalities in VCM-exposed workers. Methods: A case-control study was performed on 90 male occupationally exposed workers and 42 matched male nonexposed controls. Data were collected based on health surveillance, workplace investigation and questionnaire Survey. Total RNA and DNA were isolated from peripheral blood lymphocytes, and CYP2E1 mRNA expression was determined using RT-PCR, and the presence of CYP2E1 polymorphisms was identified based on PCR-RFLP. Results: The mRNA expression of CYP2E1 in exposed workers (0.89+/-0.46) was significantly higher than in nonexposed controls (0.61+/-0.35) (P < 0.01). Logistic regression analysis demonstrated a statistically significant association between CYP2E1 mRNA expression levels and liver abnormalities in the VCM-exposed workers (OR = 3.66, P < 0.05). The genotype frequency for CYP2E1 variants among VCM-exposed workers was not significantly different between workers with liver abnormalities and those without. Conclusions: Liver abnormalities in subjects exposed to VCM are positively associated with expression of peripheral blood lymphocyte mRNA, which is significantly increased in exposed workers compared to nonexposed controls. Therefore, CYP2E1 mRNA levels may be useful for health surveillance and protection of VCM-exposed workers.
Keywords
Humans; Men; Lymphocytes; Exposure-levels; Liver; Liver-function; Questionnaires; Workers; Work-environment; Statistical-analysis; Blood-cells; Blood-samples; Author Keywords: Vinyl chloride; Lymphocyte CYP2E1 mRNA; Polymorphism; Liver abnormality
Contact
Z-Lin Xia, Department of Occupational Health and Toxicology, Box 288, School of Public Health, Fudan University, Shanghai 200032, China
CODEN
IOMHEZ
CAS No.
9002-86-2; 75-01-4
Publication Date
20080601
Document Type
Journal Article
Email Address
zlxia@shmu.edu.cn
Funding Type
Grant
Fiscal Year
2008
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-004192
Issue of Publication
2
ISSN
0867-8383
Source Name
International Journal of Occupational Medicine and Environmental Health
State
NY
Performing Organization
Department of Environmental Health Sciences, The Mailman School of Public Health, Columbia University, New York, New York
TOP