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Mapping of phospholipids by MALDI imaging (MALDI-MSI): realities and expectations.

Authors
Sparvero-LJ; Amoscato-AA; Dixon-CE; Long-JB; Kochanek-PM; Pitt-BR; Bayir-H; Kagan-VE
Source
Chem Phys Lipids 2012 Jul; 165(5):545-562
NIOSHTIC No.
20042135
Abstract
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has emerged as a novel powerful MS methodology that has the ability to generate both molecular and spatial information within a tissue section. Application of this technology as a new type of biochemical lipid microscopy may lead to new discoveries of the lipid metabolism and biomarkers associated with area-specific alterations or damage under stress/disease conditions such as traumatic brain injury or acute lung injury, among others. However there are limitations in the range of what it can detect as compared with liquid chromatography-MS (LC-MS) of a lipid extract from a tissue section. The goal of the current work was to critically consider remarkable new opportunities along with the limitations and approaches for further improvements of MALDI-MSI. Based on our experimental data and assessments, improvements of the spectral and spatial resolution, sensitivity and specificity towards low abundance species of lipids are proposed. This is followed by a review of the current literature, including methodologies that other laboratories have used to overcome these challenges.
Keywords
Biochemistry; Lipids; Chemical-indicators; Chemical-analysis; Phospholipids; Physical-chemistry; Chemical-structure; Mass-spectrometry; Lasers; Analytical-chemistry; Analytical-instruments; Analytical-processes; Analytical-methods; Microscopy; Metabolism; Biomarkers; Brain-damage; Brain-disorders; Brain-function; Lung-disorders; Lung-function; Tissue-culture; Laboratory-techniques; Molecular-biology; Molecular-structure; Drug-interaction; Drugs; Pharmacology
Contact
Louis J. Sparvero, Center for Free Radical and Antioxidant Health, Department of Environmental and Occupational Health, University of Pittsburgh, Bridgeside Point, 100 Technology Drive, Suite 350, Pittsburgh, PA 15219-3130, USA
CODEN
CPLIA4
Publication Date
20120701
Document Type
Journal Article
Email Address
ljs39@pitt.edu
Funding Type
Grant
Fiscal Year
2012
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-008282
Issue of Publication
5
ISSN
0009-3084
Source Name
Chemistry and Physics of Lipids
State
PA; MD
Performing Organization
University of Pittsburgh at Pittsburgh
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