Mapping of phospholipids by MALDI imaging (MALDI-MSI): realities and expectations.
Sparvero-LJ; Amoscato-AA; Dixon-CE; Long-JB; Kochanek-PM; Pitt-BR; Bayir-H; Kagan-VE
Chem Phys Lipids 2012 Jul; 165(5):545-562
Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has emerged as a novel powerful MS methodology that has the ability to generate both molecular and spatial information within a tissue section. Application of this technology as a new type of biochemical lipid microscopy may lead to new discoveries of the lipid metabolism and biomarkers associated with area-specific alterations or damage under stress/disease conditions such as traumatic brain injury or acute lung injury, among others. However there are limitations in the range of what it can detect as compared with liquid chromatography-MS (LC-MS) of a lipid extract from a tissue section. The goal of the current work was to critically consider remarkable new opportunities along with the limitations and approaches for further improvements of MALDI-MSI. Based on our experimental data and assessments, improvements of the spectral and spatial resolution, sensitivity and specificity towards low abundance species of lipids are proposed. This is followed by a review of the current literature, including methodologies that other laboratories have used to overcome these challenges.
Biochemistry; Lipids; Chemical-indicators; Chemical-analysis; Phospholipids; Physical-chemistry; Chemical-structure; Mass-spectrometry; Lasers; Analytical-chemistry; Analytical-instruments; Analytical-processes; Analytical-methods; Microscopy; Metabolism; Biomarkers; Brain-damage; Brain-disorders; Brain-function; Lung-disorders; Lung-function; Tissue-culture; Laboratory-techniques; Molecular-biology; Molecular-structure; Drug-interaction; Drugs; Pharmacology
Louis J. Sparvero, Center for Free Radical and Antioxidant Health, Department of Environmental and Occupational Health, University of Pittsburgh, Bridgeside Point, 100 Technology Drive, Suite 350, Pittsburgh, PA 15219-3130, USA
Chemistry and Physics of Lipids
University of Pittsburgh at Pittsburgh