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Toxicological analysis of limonene reaction products using an in vitro exposure system.

Authors
Anderson-SE; Khurshid-SS; Mead-BJ; Lukomska-E; Wells-JR
Source
Toxicol In Vitro 2013 Mar; 27(2):721-730
NIOSHTIC No.
20041937
Abstract
Epidemiological investigations suggest a link between exposure to indoor air chemicals and adverse health effects. Consumer products contain reactive chemicals which can form secondary pollutants which may contribute to these effects. The reaction of limonene and ozone is a well characterized example of this type of indoor air chemistry. The studies described here characterize an in vitro model using an epithelial cell line (A549) or differentiated epithelial tissue (MucilAir). The model is used to investigate adverse effects following exposure to combinations of limonene and ozone. In A549 cells, exposure to both the parent compounds and reaction products resulted in alterations in inflammatory cytokine production. A one hour exposure to limonene + ozone resulted in decreased proliferation when compared to cells exposed to limonene alone. Repeated dose exposures of limonene or limonene + ozone were conducted on MucilAir tissue. No change in proliferation was observed but increases in cytokine production were observed for both the parent compounds and reaction products. Factors such as exposure duration, chemical concentration, and sampling time point were identified to influence result outcome. These findings suggest that exposure to reaction products may produce more severe effects compared to the parent compound.
Keywords
Epidemiology; Indoor-air-pollution; Exposure-levels; Pollutants; Chemical-composition; Chemical-properties; Cellular-function; Cell-function; Tissue-culture; Indoor-environmental-quality; Author Keywords: Indoor air; Limonene; Ozone; Secondary oxidation products
Contact
Stacey E. Anderson, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505
CODEN
TIVIEQ
CAS No.
138-86-3; 10028-15-6
Publication Date
20130301
Document Type
Journal Article
Email Address
sanderson4@cdc.gov
Fiscal Year
2013
NTIS Accession No.
NTIS Price
Identifying No.
B20130124
Issue of Publication
2
ISSN
0887-2333
NIOSH Division
HELD
Priority Area
Healthcare and Social Assistance; Services
Source Name
Toxicology in Vitro
State
WV; TX; GA
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