Partitioning and diffusion of parathion in human dermis.
Int J Pharm 2012 Oct; 435(1):33-37
A previously developed method employing the use of a dialysis membrane in series with human dermis tissue mounted in side-by-side diffusion cells was utilized to observe the effects of the presence of soluble proteins in the donor compartment on the measured transport parameters of parathion. In the presence of the dialysis membrane the partition coefficient was significantly lower and the diffusion coefficient significantly higher than those determined in its absence; however, the difference was less than that previously determined for the more highly protein bound compound, diclofenac. The result suggests the dialysis membrane method is important for studying permeants that are more than about 87% bound to soluble proteins in the dermis. The results are discussed in the context of a predictive model for partitioning and transport of low molecular weight solutes in human dermis.
Diffusion-analysis; Humans; Men; Women; Proteins; Metabolism; Skin; Tissue-culture;
Author Keywords: Albumin; Dermis; Diffusion; Drug transport; Permeability; Protein binding; Membrane transport; Tissue partition; Mathematical model
James L. Winkle College of Pharmacy, University of Cincinnati Academic Health Center, P.O. Box 670004, Cincinnati, OH 45267-0004
International Journal of Pharmaceutics
University of Cincinnati