Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Cochlear base-apex differences in cell-death pathways following exposure to low-frequency noise.

Authors
Lee-SC; Bohne-BA; Harding-GW
Source
Open Otorhinolaryngol J 2008 Feb; 2(1):29-43
NIOSHTIC No.
20041559
Abstract
Objective: To determine what death pathways are followed by noise-damaged outer hair cells (OHCs). Methods: Chinchillas were exposed to a 0.5-kHz octave band of noise for 24 h. Administration of trypan blue in vivo was used to stain nuclei in cells with leaky plasma membranes. After removing unbound dye, the cochleae were fixed, plasticembedded then dissected to obtain flat preparations of the entire organ of Corti (OC). Using morphological criteria, dying and injured OHCs were classified as following an apoptotic or oncotic death pathway, or a pathway that was neither apoptotic nor oncotic. Results: The exposure caused scattered loss of OHCs in the apical OC and, sometimes, focal hair-cell losses in the basal OC. In the apical OC, many dying OHCs appeared to be following a death pathway that was different from apoptosis and oncosis. In the basal OC, oncotic OHCs were found at the edges of focal lesions. Conclusion: The morphological appearance of dying OHCs in the apical OC was distinct from those following an apoptotic or oncotic death pathway, suggesting that these OHCs were following a previously unidentified death pathway.
Keywords
Noise; Noise-exposure; Noise-levels; Noise-measurement; Exposure-levels; Laboratory-animals; Animals; Cell-damage; Noise-induced-hearing-loss; Hazards; Audiological-testing; Author Keywords: Cell-death pathways; noise damage; chinchilla; histopathology
Contact
Barbara A. Bohne, Department of Otolaryngology - Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO 63110
CODEN
OOJPD7
Publication Date
20080201
Document Type
Journal Article
Email Address
bohneb@ent.wustl.edu
Fiscal Year
2008
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-003973
Issue of Publication
1
ISSN
1874-4281
Source Name
The Open Otorhinolaryngology Journal
State
MO
Performing Organization
Washington University, St. Louis
TOP