Single cell gel electrophoresis assay (comet assay) for evaluating manoparticles-induced DNA damage in cells.
Bowman-L; Castranova-V; Ding-M
Nanoparticles in biology and medicine: methods and protocols, methods in molecular biology. Soloviev M, ed., New York: Springer, 2012 Jan; 906:415-422
Comet assay provides a rapid and reliable way to screen genotoxic effects of a wide variety of nanoparticles. Single cell gel electrophoresis assay (SCGE) or comet assay gained in popularity as a standard technique and is widely used for testing novel particles or chemicals for genotoxicity, monitoring environmental contamination with genotoxins, human biomonitoring and molecular epidemiology, and fundamental research in DNA damage and repair. Here, we describe the method of alkaline comet assay to detect nanoparticle-induced DNA damage.
Nanotechnology; Cell-biology; Cell-function; Genotoxicity; Particulates; Chemical-composition; DNA-damage; Alkalies;
Author Keywords: Nanoparticles; DNA damage; Genotoxicity; Reactive oxygen species; Comet assay
Book or book chapter
Nanoparticles in biology and medicine: methods and protocols, methods in molecular biology