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Single cell gel electrophoresis assay (comet assay) for evaluating manoparticles-induced DNA damage in cells.

Authors
Bowman-L; Castranova-V; Ding-M
Source
Nanoparticles in biology and medicine: methods and protocols, methods in molecular biology. Soloviev M, ed., New York: Springer, 2012 Jan; 906:415-422
NIOSHTIC No.
20041170
Abstract
Comet assay provides a rapid and reliable way to screen genotoxic effects of a wide variety of nanoparticles. Single cell gel electrophoresis assay (SCGE) or comet assay gained in popularity as a standard technique and is widely used for testing novel particles or chemicals for genotoxicity, monitoring environmental contamination with genotoxins, human biomonitoring and molecular epidemiology, and fundamental research in DNA damage and repair. Here, we describe the method of alkaline comet assay to detect nanoparticle-induced DNA damage.
Keywords
Nanotechnology; Cell-biology; Cell-function; Genotoxicity; Particulates; Chemical-composition; DNA-damage; Alkalies; Author Keywords: Nanoparticles; DNA damage; Genotoxicity; Reactive oxygen species; Comet assay
Publication Date
20120101
Document Type
Book or book chapter
Editors
Soloviev-M
Fiscal Year
2012
NTIS Accession No.
NTIS Price
ISBN No.
9781617799525
Identifying No.
B08012012
NIOSH Division
HELD
Priority Area
Construction; Manufacturing
Source Name
Nanoparticles in biology and medicine: methods and protocols, methods in molecular biology
State
WV
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