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Citrullination of proteins: a common posttranslational modification pathway induced by different nanoparticles in vitro and in vivo.

Authors
Mohamed-BM; Verma-NK; Davies-AM; McGowan-A; Staunton-KC; Prina-Mello-A; Kelleher-D; Botting-CH; Causey-CP; Thompson-PR; Pruijn-GJ; Kisin-ER; Tkach-AV; Shvedova-AA; Volkov-Y
Source
Nanomed 2012 Aug; 7(8):1181-1195
NIOSHTIC No.
20040892
Abstract
Aim: Rapidly expanding manufacture and use of nanomaterials emphasize the requirements for thorough assessment of health outcomes associated with novel applications. Post-translational protein modifications catalyzed by Ca2+-dependent peptidylargininedeiminases have been shown to trigger immune responses including autoantibody generation, a hallmark of immune complexes deposition in rheumatoid arthritis. Therefore, the aim of the study was to assess if nanoparticles are able to promote protein citrullination. Materials & methods: Human A549 and THP-1 cells were exposed to silicon dioxide, carbon black or single-walled carbon nanotubes. C57BL/6 mice were exposed to respirable single-walled carbon nanotubes. Protein citrullination, peptidylargininedeiminases activity and target proteins were evaluated. Results: The studied nanoparticles induced protein citrullination both in cultured human cells and mouse lung tissues. Citrullination occurred via the peptidylargininedeiminase-dependent mechanism. Cytokeratines 7, 8, 18 and plectins were identified as intracellular citrullination targets. Conclusion: Nanoparticle exposure facilitated post-translational citrullination of proteins.
Keywords
Nanotechnology; Immune-reaction; Immune-system; Immunology; Proteins; Protein-biosynthesis; Protein-biochemistry; In-vivo-studies; In-vitro-studies; Cellular-reactions; Cellular-function; Author Keywords: autoimmunity; high content analysis; immune system; inflammation; nanomaterial; nanoparticle; peptidylargininedeiminase; post-translational modification; protein citrullination; rheumatoid arthritis
Contact
Anna A Shvedova, Health Effects Laboratory Division, NIOSH, Morgantown, WV, USA
CODEN
NLUKAC
CAS No.
1333-86-4; 7631-86-9
Publication Date
20120801
Document Type
Journal Article
Email Address
ats1@cdc.gov
Fiscal Year
2012
NTIS Accession No.
NTIS Price
Identifying No.
B06202012
Issue of Publication
8
ISSN
1743-5889
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
Nanomedicine
State
WV
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