Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Development of pulmonary tolerance in mice exposed to zinc oxide fumes.

Authors
Wesselkamper-SC; Chen-LC; Gordon-T
Source
Toxicol Sci 2001 Mar; 60(1):144-151
NIOSHTIC No.
20040772
Abstract
As a result of repeated exposures to inhaled toxicants such as zinc oxide (ZnO), numerous individuals acquire tolerance to the exposures and display reduced symptoms. To ascertain whether tolerance is developed in an animal model, NIH-Swiss mice were exposed to 1.0 mg/m(3) ZnO for 1, 3, or 5 days (1X, 3X, or 5X), and polymorphonuclear leukocyte (PMN) and protein levels in bronchoalveolar lavage (BAL) were measured. Mice acquired tolerance to neutrophil infiltration into the lungs, as total PMNs returned near baseline in 5X-exposed animals as compared to that of the 1X exposure group (1X = 2.7 +/- 0.4 x 10(4), 5X = 0.2 +/- 0.1 x 10(4), mean +/- SE, p < 0.05). Development of tolerance to changes in lavageable protein, however, was not observed (1X = 313 +/- 29 microg/ml, 5X = 684 +/- 71 microg/ml, p < 0.05). Tolerance to PMN influx did not persist following re-exposure to ZnO after 5 days of rest. In contrast to ZnO exposure, following single and repeated exposure to aerosolized endotoxin there was development of tolerance to protein in BAL (1X = 174 +/- 71 microg/ml, 5X = 166 +/- 14 microg/ml, p > 0.05), but not to PMN influx (1X = 5.5 +/- 1.7 x 10(4), 13.9 +/- 1.7 x 10(4), p < 0.05). Induction of lung metallothionein (MT) was also observed in mice exposed once or repeatedly exposed to ZnO, suggesting that MT may play a role in its molecular mechanism.
Keywords
Zinc-compounds; Inhalants; Clinical-symptoms; Tolerance-threshold; Laboratory-animals; Laboratory-testing; Exposure-levels; Exposure-assessment; Leukocytes; Alveolar-cells; Pulmonary-function; Pulmonary-system; Lung-irritants; Fumes; Metal-fumes; Toxic-vapors; Toxins; Proteins; Endotoxins; Neutrophils; Adaptation; Metalloproteins; Metal-fume-fever; Author Keywords: zinc-oxide; tolerance; Metallothionein; metal fume fever; endotoxin; adaptation
Contact
Terry Gordon, Department of Environmental Medicine, New York University School of Medicine, 57 Old Forge Road, Tuxedo, New York 10987, USA
CODEN
TOSCF2
CAS No.
7440-66-6; 1314-13-2
Publication Date
20010301
Document Type
Journal Article
Email Address
gordont@env.med.nyu.edu
Funding Amount
697800
Funding Type
Grant
Fiscal Year
2001
NTIS Accession No.
NTIS Price
Identifying No.
Grant-number-R01-OH-002987
Issue of Publication
1
ISSN
1096-6080
Priority Area
Pulmonary System Disorders
Source Name
Toxicological Sciences
State
NY
Performing Organization
Yale University, New Haven, Connecticut
TOP