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Dispersal state of multiwalled carbon nanotubes elicits profibrogenic cellular responses that correlate with fibrogenesis biomarkers and fibrosis in the murine lung.

Authors
Wang-X; Xia-T; Addo Ntim-S; Ji-Z; Lin-S; Meng-H; Chung-C-H; George-S; Zhang-H; Wang-M; Li-N; Yang-Y; Castranova-V; Mitra-S; Bonner-JC; Nel-AE
Source
ACS Nano 2011 Dec; 5(12):9772-9787
NIOSHTIC No.
20040211
Abstract
We developed a dispersal method for multiwalled carbon nanotubes (MWCNTs) that allows quantitative assessment of dispersion on profibrogenic responses in tissue culture cells and in mouse lung. We demonstrate that the dispersal of as-prepared (AP), purified (PD), and carboxylated (COOH) MWCNTs by bovine serum albumin (BSA) and dipalmitoylphosphatidylcholine (DPPC) influences TGF-▀1, PDGF-AA, and IL-1▀ production in vitro and in vivo. These biomarkers were chosen based on their synergy in promoting fibrogenesis and cellular communication in the epithelial-mesenchymal cell trophic unit in the lung. The effect of dispersal was most noticeable in AP- and PD-MWCNTs, which are more hydrophobic and unstable in aqueous buffers than hydrophilic COOH-MWCNTs. Well-dispersed AP- and PD-MWCNTs were readily taken up by BEAS-2B, THP-1 cells, and alveolar macrophages (AM) and induced more prominent TGF-▀1 and IL-1▀ production in vitro and TGF-▀1, IL-1▀, and PDGF-AA production in vivo than nondispersed tubes. Moreover, there was good agreement between the profibrogenic responses in vitro and in vivo as well as the ability of dispersed tubes to generate granulomatous inflammation and fibrosis in airways. Tube dispersal also elicited more robust IL-1▀ production in THP-1 cells. While COOH-MWCNTs were poorly taken up in BEAS-2B and induced little TGF-▀1 production, they were bioprocessed by AM and induced less prominent collagen deposition at sites of nongranulomatous inflammation in the alveolar region. Taken together, these results indicate that the dispersal state of MWCNTs affects profibrogenic cellular responses that correlate with the extent of pulmonary fibrosis and are of potential use to predict pulmonary toxicity.
Keywords
Nanotechnology; Quantitative-analysis; Lung-cells; In-vitro-study; Fibrogenesis; Fibrogenicity; Respiratory-system-disorders; Lung-disorders; Pulmonary-system-disorders; Author Keywords: multiwalled carbon nanotubes (MWCNTs); dispersion; TGF-▀1; PDGF-AA; IL-1▀; collagen; lung fibrosis
Contact
Andre┤ E. Nel, California NanoSystems Institute, University of California, Los Angeles, California 90095, United States
CODEN
ANCAC3
Publication Date
20111227
Document Type
Journal Article
Email Address
anel@mednet.ucla.edu
Fiscal Year
2012
NTIS Accession No.
NTIS Price
Issue of Publication
12
ISSN
1936-0851
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
ACS Nano
State
CA; NJ; NC; WV
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