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Eurasian-origin gene segments contribute to the transmissibility, aerosol release, and morphology of the 2009 pandemic H1N1 influenza virus.

Authors
Lakdawala-SS; Lamirande-EW; Suguitan-AL Jr.; Wang-W; Santos-CP; Vogel-L; Matsuoka-Y; Lindsley-WG; Jin-H; Subbarao-K
Source
PLoS Pathog 2011 Dec; 7(12):e1002443
NIOSHTIC No.
20040154
Abstract
The epidemiological success of pandemic and epidemic influenza A viruses relies on the ability to transmit efficiently from person-to-person via respiratory droplets. Respiratory droplet (RD) transmission of influenza viruses requires efficient replication and release of infectious influenza particles into the air. The 2009 pandemic H1N1 (pH1N1) virus originated by reassortment of a North American triple reassortant swine (TRS) virus with a Eurasian swine virus that contributed the neuraminidase (NA) and M gene segments. Both the TRS and Eurasian swine viruses caused sporadic infections in humans, but failed to spread from person-to-person, unlike the pH1N1 virus. We evaluated the pH1N1 and its precursor viruses in a ferret model to determine the contribution of different viral gene segments on the release of influenza virus particles into the air and on the transmissibility of the pH1N1 virus. We found that the Eurasian-origin gene segments contributed to efficient RD transmission of the pH1N1 virus likely by modulating the release of influenza viral RNA-containing particles into the air. All viruses replicated well in the upper respiratory tract of infected ferrets, suggesting that factors other than viral replication are important for the release of influenza virus particles and transmission. Our studies demonstrate that the release of influenza viral RNA-containing particles into the air correlates with increased NA activity. Additionally, the pleomorphic phenotype of the pH1N1 virus is dependent upon the Eurasian-origin gene segments, suggesting a link between transmission and virus morphology. We have demonstrated that the viruses are released into exhaled air to varying degrees and a constellation of genes influences the transmissibility of the pH1N1 virus.
Keywords
Humans; Men; Children; Immune-reaction; Immune-system; Immune-system-disorders; Immunology; Infectious-diseases; Respiration; Respiratory-infections; Respiratory-system-disorders; Pulmonary-function; Pulmonary-system; Pulmonary-system-disorders; Pulmonary-congestion; Air-contamination; Airborne-particles; Exposure-levels; Exposure-limits; Vapors; Particulates; Models; Laboratory-animals; Animals; Viral-diseases; Viral-infections
CODEN
PPLACN
Publication Date
20111229
Document Type
Journal Article
Email Address
kanta.subbarao@nih.gov
Editors
Fouchier-RAM
Fiscal Year
2012
NTIS Accession No.
NTIS Price
Identifying No.
B01182012
Issue of Publication
12
ISSN
1553-7374
NIOSH Division
HELD
Priority Area
Healthcare and Social Assistance
Source Name
Public Library of Science Pathogens
State
CA; MD; WV
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