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Evaluation of the interactions between multiwalled carbon nanotubes and Caco-2 cells.

Authors
Clark-KA; O'Driscoll-C; Cooke-CA; Smith-BA; Wepasnick-K; Fairbrother-DH; Lees-PSJ; Bressler-JP
Source
J Toxicol Environ Health, A 2012 Jan; 75(1):25-35
NIOSHTIC No.
20040114
Abstract
The aim of this study was to determine whether multiwalled carbon nanotubes (MWNCT) are taken up by and are toxic to human intestinal enterocytes using the Caco-2 cell model. Caco-2 cells were exposed to 50 g/ml MWCNT (oxidized or pristine) for 24 h, and experiments were repeated in the presence of 2.5 mg/L natural organic matter. Cells displayed many of the properties that characterize enterocytes, such as apical microvilli, basolateral basement membrane, and glycogen. The cell monolayers also displayed tight junctions and electrical resistance. Exposure to pristine and oxidized MWCNT, with or without natural organic matter, did not markedly affect viability, which was assessed by measuring activity of released lactate dehydrogenase (LDH) and staining with propidium iodide. Ultrastructural analysis revealed some damage to microvilli colocalized with the MWCNT; however, neither type of MWCNT was taken up by Caco-2 cells. In contrast, pristine and oxidized MWCNT were taken up by the macrophage RAW 264.7 line. Our study suggests that intestinal enterocytes cells do not take up MWCNT.
Keywords
Nanotechnology; Intestinal-cells; Toxic-effects; Exposure-assessment; Exposure-levels; Exposure-methods; Humans; Cell-alteration; Cell-cultures; Cell-metabolism; Cellular-reactions; Cytotoxic-effects; Organic-compounds; Membrane-dysfunction; Metabolism; Ultrastructure; Drug-interaction; Oxidation
Contact
Joseph P. Bressler, Kennedy Krieger Institute, 707 North Broadway, Baltimore MD 21215, USA
CODEN
JTEHD6
CAS No.
7440-44-0; 25535-16-4
Publication Date
20120101
Document Type
Journal Article
Email Address
bressler@kennedykrieger.org
Funding Type
Grant
Fiscal Year
2012
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-T42-OH-008428; B01182012
Issue of Publication
1
ISSN
1528-7394
Source Name
Journal of Toxicology and Environmental Health, Part A: Current Issues
State
MD
Performing Organization
Johns Hopkins University
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