Intratracheal instillation of cerium oxide nanoparticles induces hepatic toxicity in male Sprague-Dawley rats.
Nalabotu-SK; Kolli-MB; Triest-WE; Ma-JY; Manne-NDPK; Katta-A; Addagarla-HS; Rice-KM; Blough-ER
Int J Nanomed 2011 Oct; 2011(6):2327-2335
Background: Cerium oxide (CeO(2)) nanoparticles have been posited to have both beneficial and toxic effects on biological systems. Herein, we examine if a single intratracheal instillation of CeO(2) nanoparticles is associated with systemic toxicity in male Sprague-Dawley rats. Methods and results: Compared with control animals, CeO(2) nanoparticle exposure was associated with increased liver ceria levels, elevations in serum alanine transaminase levels, reduced albumin levels, a diminished sodium-potassium ratio, and decreased serum triglyceride levels (P < 0.05). Consistent with these data, rats exposed to CeO(2) nanoparticles also exhibited reductions in liver weight (P < 0.05) and dose-dependent hydropic degeneration, hepatocyte enlargement, sinusoidal dilatation, and accumulation of granular material. No histopathological alterations were observed in the kidney, spleen, and heart. Analysis of serum biomarkers suggested an elevation of acute phase reactants and markers of hepatocyte injury in the rats exposed to CeO(2) nanoparticles. Conclusion: Taken together, these data suggest that intratracheal instillation of CeO(2) nanoparticles can result in liver damage.
Nanotechnology; Particulates; Cerium-compounds; Liver-damage; Liver-disorders; Animals; Laboratory-animals; Laboratory-testing; Animal-studies; Serological-techniques; Serology; Biological-effects; Toxicology; Liver-function; Dose-response; Hepatocytes; Hepatotoxicity; Biomarkers;
Author Keywords: cerium oxide nanoparticles; systemic toxicity; hepatic toxicity; hydropic
Eric Blough, Center for Diagnostic Nanosystems, Robert C Byrd Biotechnology Science Center, 1700 3rd Ave, Marshall University, Huntington, WV 25755-1090, USA
Transportation, Warehousing and Utilities
International Journal of Nanomedicine