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Antioxidant c-FLIP inhibits Fas ligand-induced NF-kB activation in a phosphatidylinositol 3-kinase/Akt-dependent manner.

Authors
Iyer-AKV; Azad-N; Talbot-S; Stehlik-C; Lu-B; Wang-L; Rojanasakul-Y
Source
J Immunol 2011 Sep; 187(6):3256-3266
NIOSHTIC No.
20039755
Abstract
Fas ligand (FasL) belongs to the TNF family of death ligands, and its binding to the FasR leads to activation of several downstream signaling pathways and proteins, including NF-kB and PI3K/Akt. However, it is not known whether cross-talk exists between NF-?B and PI3K/Akt in the context of FasL signaling. We demonstrate using both human renal epithelial 293T cells and Jurkat T-lymphocyte cells that although FasL activates both Akt and NF-kB, Akt inhibits FasL-dependent NF-kB activity in a reactive oxygen species-dependent manner. Cellular FLICE-inhibitory protein (c-FLIP), an antioxidant and an important component of the death-inducing signaling complex, also represses NF-?B upstream of the regulatory IkB kinase-y protein subunit in the NF-kB signaling pathway, and positive cross-talk exists between Akt and c-FLIP in the context of inhibition of FasL-induced NF-kB activity. The presence of two death effector domains of c-FLIP and S-nitrosylation of its caspase-like domain were found to be important for mediating c-FLIP-dependent downregulation of NF-kB activity. Taken together, our study reveals a novel link between NF-kB and PI3K/Akt and establishes c-FLIP as an important regulator of FasL-mediated cell death.
Keywords
Cell-biology; Cell-cultures; Cell-damage; Cell-function; Proteins; Humans; Antioxidants; Antioxidation; Immunology; Immune-reaction; Immunochemistry
Contact
Dr. Anand Krishnan V. Iyer, Department of Pharmaceutical Sciences, Hampton University, Hampton, VA 23668
CODEN
JOIMA3
Publication Date
20110915
Document Type
Journal Article
Email Address
anand.iyer@hamptonu.edu
Fiscal Year
2011
NTIS Accession No.
NTIS Price
Identifying No.
B10122011
Issue of Publication
6
ISSN
0022-1767
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
The Journal of Immunology
State
IL; MA; VA; WV
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