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Spectroelectrochemical sensing of pyrene metabolites 1-hydroxypyrene and 1-hydroxypyrene-glucuronide.

Authors
Wilson-RA; Seliskar-CJ; Talaska-G; Heineman-WR
Source
Anal Chem 2011 May; 83(10):3725-3729
NIOSHTIC No.
20039537
Abstract
Spectroelectrochemical sensing in an optically transparent thin layer electrode (OTTLE) cell was used for detecting the polycyclic aromatic hydrocarbon (PAH) biomarkers 1-hydroxypyrene (1-pyOH) and 1-hydroxypyrene-glucuronide (1-pyOglu) in phosphate buffer and artificial urine. This approach uses selective electrochemical modulation of a fluorescence signal by sequentially oxidizing the analytes in an OTTLE cell to distinguish between their overlapping fluorescence spectra. This technique allows for complete oxidation and signal modulation in approximately 15 min for each analyte; a mixture of 1-pyOH and its glucuronic acid conjugate can be analyzed in 30 min. Calibration curves consisting of the fluorescence change vs analyte concentration for 1-pyOH and 1-pyOglu yielded linear ranges from 10 nM to 1 M and from 1 nM to 1 M, respectively. With the use of these results, the calculated limits of detection were determined to be 1 10(-8) M for 1-pyOH and 9 10(-11) M for 1-pyOglu.
Keywords
Biomarkers; Cell-biology; Cell-function; Cellular-reactions; Electrochemical-analysis; Mathematical-models; Physical-reactions; Physiological-effects; Physiological-response; Statistical-analysis
Contact
William R. Heineman, Department of Chemistry, University of Cincinnati, 301 Clifton Court, Cincinnati, Ohio 45221-0172
CODEN
ANCHAM
Publication Date
20110515
Document Type
Journal Article
Email Address
william.heineman@uc.edu
Funding Type
Grant
Fiscal Year
2011
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-009652
Issue of Publication
10
ISSN
0003-2700
Source Name
Analytical Chemistry
State
OH
Performing Organization
University of Cincinnati
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