Allergic potential and immunotoxicity induced by topical application of 1-chloro-4-(trifluoromethyl)benzene (PCBTF) in a murine model.
Franko-J; Jackson-LG; Meade-BJ; Anderson-SE
J Allergy 2011 Mar; 2011:238513
The purpose of the studies in this paper was to evaluate the allergic potential, immunotoxicity, and irritancy of the occupationally relevant chemical, 1-chloro-4-(trifluoromethyl)benzene, also known as parachlorobenzotrifluoride (PCBTF), following dermal exposure in a murine model. Evaluation of the sensitization potential, conducted using the local lymph node assay (LLNA) at concentrations ranging from 50% to 100%, identified a dose-dependent increase in lymphocyte proliferation with a calculated EC3 value of 53.1%. While no elevations in total or specific IgE were observed after exposure to any concentration of the chemical, significant increases in IFN-y protein production by stimulated draining lymphoid cells were observed, indicating a T-cell-mediated response. Dermal exposure to PCBTF was not found to alter the immune response to a T-cell-dependant antigen. These results demonstrate that PCBTF has the potential to induce allergic sensitization following dermal exposure and based on LLNA results would be classified as a weak sensitizer.
Chemical-hypersensitivity; Chemical-properties; Exposure-assessment; Exposure-levels; Immune-reaction; Immune-system-disorders; Immunologic-disorders; Immunotoxins; Lymphocytes; Quantitative-analysis; Risk-analysis; Risk-factors; Safety-education; Skin; Skin-exposure; Statistical-analysis; Toxic-effects; Work-environment; Workplace-studies
Jennifer Franko, Health Effects Laboratory Division, National Institute for Occupational Safety and Health (NIOSH), 1095 Willowdale Drive, Morgantown, WV 26505-2888
Journal of Allergy