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A novel immunochromatographic electrochemical biosensor for highly sensitive and selective detection of trichloropyridinol, a biomarker of exposure to chlorpyrifos.

Authors
Wang-L; Lu-D; Wang-J; Du-D; Zou-Z; Wang-H; Smith-JN; Timchalk-C; Liu-F; Lin-Y
Source
Biosens Bioelectron 2011 Feb; 26(6):2835-2840
NIOSHTIC No.
20038748
Abstract
We present a novel portable immunochromatographic electrochemical biosensor (IEB) for simple, rapid, and sensitive biomonitoring of trichloropyridinol (TCP), a metabolite biomarker of exposure to organophosphorus insecticides. Our new approach takes the advantage of immunochromatographic test strip for a rapid competitive immunoreaction and a disposable screen-printed carbon electrode for a rapid and sensitive electrochemical analysis of captured HRP labeling. Several key experimental parameters (e.g. immunoreaction time, the amount of HRP labeled TCP, concentration of the substrate for electrochemical measurements, and the blocking agents for the nitrocellulose membrane) were optimized to achieve a high sensitivity, selectivity and stability. Under optimal conditions, the IEB has demonstrated a wide linear range (0.1-100 ng/ml) with a detection limit as low as 0.1 ng/ml TCP. Furthermore, the IEB has been successfully applied for biomonitoring of TCP in the rat plasma samples with in vivo exposure to organophosphorus insecticides like Chlorpyrifos-oxon (CPF-oxon). The IEB thus opens up new pathways for designing a simple, rapid, clinically accurate, and quantitative tool for TCP detection, as well as holds a great promise for in-field screening of metabolite biomarkers, e.g., TCP, for humans exposed to organophosphorus insecticides.
Keywords
Sensitivity-testing; Biological-monitoring; Immunochemistry; Chromatographic-analysis; Biomarkers; Metabolites; Organo-phosphorus-compounds; Organo-phosphorus-pesticides; Insecticides; Electrochemical-analysis; Immune-reaction; In-vivo-study; Exposure-assessment; Enzyme-activity; Enzyme-complexes; Peroxidases; Acetylcholinesterase; Antibody-response; Author Keywords: Immunochromatographic electrochemical biosensor; Biomarker; Organophosphorus insecticides; Trichloropyridinol; Competitive immunoreaction
CODEN
BBIOE4
CAS No.
6515-38-4; 2921-88-2; 5598-15-2; 5598-13-0
Publication Date
20110215
Document Type
Journal Article
Funding Type
Grant
Fiscal Year
2011
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-008173
Issue of Publication
6
ISSN
0956-5663
Source Name
Biosensors and Bioelectronics
State
WA
Performing Organization
Battelle Pacific Northwest Laboratories
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