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Role of germination in murine airway CD8(+) T-cell responses to Aspergillus conidia.

Authors
Templeton-SP; Buskirk-AD; Law-B; Green-BJ; Beezhold-DH
Source
PLoS One 2011 Apr; 6(4):e18777
NIOSHTIC No.
20038688
Abstract
Pulmonary exposure to Aspergillus fumigatus has been associated with morbidity and mortality, particularly in immunocompromised individuals. A. fumigatus conidia produce beta-glucan, proteases, and other immunostimulatory factors upon germination. Murine models have shown that the ability of A. fumigatus to germinate at physiological temperature may be an important factor that facilitates invasive disease. We observed a significant increase in IFN-gamma-producing CD8(+) T cells in bronchoalveolar lavage fluid (BALF) of immunocompetent mice that repeatedly aspirated A. fumigatus conidia in contrast to mice challenged with A. versicolor, a species that is not typically associated with invasive, disseminated disease. Analysis of tissue sections indicated the presence of germinating spores in the lungs of mice challenged with A. fumigatus, but not A. versicolor. Airway IFN-gamma(+)CD8(+) T-cells were decreased and lung germination was eliminated in mice that aspirated A. fumigatus conidia that were formaldehyde-fixed or heat-inactivated. Furthermore, A. fumigatus particles exhibited greater persistence in the lungs of recipient mice when compared to non-viable A. fumigatus or A. versicolor, and this correlated with increased maintenance of airway memory-phenotype CD8(+) T cells. Therefore, murine airway CD8(+) T cell-responses to aspiration of Aspergillus conidia may be mediated in part by the ability of conidia to germinate in the host lung tissue. These results provide further evidence of induction of immune responses to fungi based on their ability to invade host tissue.
Keywords
Bioactivation; Fungi; Fungicides; Immune-reaction; Immune-system; Immune-system-disorders; Laboratory-animals; Laboratory-testing; Lung-disorders; Microbiology; Particulates; Pathogenesis; Physiological-effects; Physiological-response; Pulmonary-system; Pulmonary-system-disorders; Respiratory-equipment; Respiratory-irritants; Respiratory-system-disorders; Microorganisms
Contact
Steven P. Templeton, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV 26505 USA
CODEN
POLNCL
Publication Date
20110413
Document Type
Journal Article
Email Address
STempleton@cdc.gov
Fiscal Year
2011
NTIS Accession No.
NTIS Price
Issue of Publication
4
ISSN
1932-6203
NIOSH Division
HELD
Priority Area
Agriculture, Forestry and Fishing; Services
Source Name
Public Library of Science One
State
WV
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