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Pre-sampling contamination of filters used in measurements of airborne (1 --> 3)-beta-D-glucan based on glucan-specific Limulus amebocyte lysate assay.

Authors
Shogren-ES; Park-J-H
Source
J Environ Monit 2011 Apr; 13(4):1082-1087
NIOSHTIC No.
20038661
Abstract
Air sampling for (1 --> 3)-beta-D-glucan may be a good method for assessing inhalation exposure to airborne fungi. Pre-sampling contamination of filter media used for sampling (1 --> 3)-beta-D-glucan may lead to substantial exposure measurement errors. Using the Limulus amebocyte lysate assay, we tested for pre-sampling levels of (1 --> 3)-beta-D-glucan on three types of filters-mixed cellulose ester (MCE) [1 brand], glass fiber (GF)[1 brand], and polycarbonate (PC)[5 brands]. Levels of (1 --> 3)-beta-D-glucan on MCE filters exceeded 4586.1 pg per filter. Levels on GF filters averaged 135.3 (+/- 28.9) pg per filter (range = 94.8-160.4 pg per filter) and levels on PC filters averaged 152.4 (+/- 236.1) pg per filter (range = non-detectable-1760.7 pg per filter). Efforts to clean MCE and GF filters were unfeasible or unsuccessful. Sonicating PC filters for two hours in ethanol, followed by a wash in pyrogen-free water, effectively eliminated measured levels of (1 --> 3)-beta-D-glucan on four brands of PC filters, as compared to untreated PC filters. This pretreatment process did not appear to physically damage the PC filters. Air sampling results highlighted the potentially problematic contamination of untreated PC filters. Ensuring that sampling media are free of (1 --> 3)-beta-D-glucan before sampling is crucial to accurately measure levels of (1 --> 3)-beta-D-glucan exposure, especially in environments where levels of (1 --> 3)-beta-D-glucan are low.
Keywords
Inhalation-studies; Exposure-assessment; Air-sampling; Air-contamination; Airborne-particles; Fungi; Filters; Bioassays; Cellulose-fibers; Pretreatment; Air-samplers; Equipment-reliability; Cell-function; Respiratory-irritants; Respiratory-system-disorders; Polysaccharides; Dust-inhalation; Dust-sampling; Measurement-equipment
Contact
Ju-Hyeong Park, National Institute for Occupational Safety and Health, Division of Respiratory Disease Studies, 1095 Willowdale Road, Morgantown, West Virginia, 26505, USA
CODEN
JEMOFW
Publication Date
20110401
Document Type
Journal Article
Email Address
gzp8@cdc.gov
Fiscal Year
2011
NTIS Accession No.
NTIS Price
Issue of Publication
4
ISSN
1464-0325
NIOSH Division
DRDS
Priority Area
Services
Source Name
Journal of Environmental Monitoring
State
WV
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