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Oxidative lipidomics reveals selective, but not random, pulmonary phosphilipid peroxidation after inhalation of carbon nanotubes.

Authors
Tyurina-Y; Tyurin-V; Sparvero-L; Amoscato-A; Kapralova-V; Kisin-E; Murray-A; Shi-J; Fadeel-B; Shvedova-A; Kagan-V
Source
Toxicologist 2011 Mar; 120(Suppl 2):252
NIOSHTIC No.
20038512
Abstract
It is commonly accepted that lipid peroxidation is a mechanism of cell/tissue damage and death by nanoparticles, including single walled carbon nanotubes (SWCNT).We performed global ipidomics/oxidative lipidomics analysis of all molecular species in major classes of phospholipids and established that random free radical peroxidation does not happen in the lung of mice at 3 different time-points after SWCNT inhalation. C57BL/6 mice were exposed to SWCNT via inhalation (5mg/m3, whole body inhalation for 4 consecutive days, 5 h/day) and scarified 1, 7 and 28 days thereafter.We found that two most abundant and susceptible phospholipids - phosphatidylcholine and phosphatidylethanolamine - that constitute more than 75% of total phospholipids, do not undergo peroxidation at all. In addition, we demonstrated that three relatively minor anionic phospholipids - cardiolipin, phosphatidylinositol, and phosphatidylserine get oxidized in a very selective way: only few molecular species out of many in each class (10 out of 25 for CL, 3 out of 5 for PI, 1 out of 7 for PS) were converted into oxygenated species. Moreover, the selectivity was also detected towards fatty acids of the respective oxidized anionic phospholipids (not most polyunsaturated fatty acids but most abundant). We speculate that SWCNT-induced phospholipid peroxidation in lung is involved in the production of selective precursors of pro-resolving mediators of inflammation rather than in non-specific oxidative damage.
Keywords
Cell-damage; Cell-function; Cellular-reactions; Environmental-control; Injury-prevention; Laboratory-animals; Laboratory-testing; Lung-irritants; Molecular-biology; Molecular-structure; Nanotechnology; Oxidative-metabolism; Oxidative-processes; Peroxidases; Pulmonary-function; Pulmonary-system; Quantitative-analysis; Respiratory-hypersensitivity; Respiratory-irritants; Respiratory-system-disorders; Risk-analysis; Risk-factors; Statistical-analysis; Tissue-disorders; Toxins
CAS No.
7440-44-0
Publication Date
20110301
Document Type
Abstract
Funding Type
Grant
Fiscal Year
2011
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-008282
ISSN
1096-6080
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC
State
DC; WV; PA
Performing Organization
University of Pittsburgh at Pittsburgh
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