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Comparison of air sampling methods for aerosolized spores of B. anthracis sterne.

Authors
Estill-CF; Baron-PA; Beard-JK; Hein-MJ; Larsen-LD; Deye-GJ; Rose-L; Hodges-L
Source
J Occup Environ Hyg 2011 Mar; 8(3):179-186
NIOSHTIC No.
20038406
Abstract
Bacillus anthracis Sterne spores were aerosolized within a chamber at concentrations ranging from 110(3) to 1.710(4) spores per cubic meter of air (particles (p)/m(3)) to compare three different sampling methods: Andersen samplers, gelatin filters, and polytetrafluoroethylene (PTFE) membrane filters. Three samples of each type were collected during each of 19 chamber runs. Chamber concentration was determined by an aerodynamic particle sizer (APS) for the size range of 1.114-1.596 m. Runs were categorized (low, medium, and high) based on tertiles of the APS estimated air concentrations. Measured air concentrations and recovery efficiency [ratio of the measured (colony forming units (CFU)/m(3)) to the APS estimated (particles/m(3)) air concentrations] for the sampling methods were compared using mixed-effects regression models. Limits of detection for each method were estimated based on estimated recovery efficiencies. Mean APS estimated air concentrations were 1600 particles/m(3), 4100 particles/m(3), and 9100 particles/m(3) at the low, medium, and high tertiles, respectively; coefficient of variation (CV) ranged from 25 to 40%. Statistically significant differences were not observed among the three sampling methods. At the high and medium tertiles, estimated correlations of measured air concentration (CFU/m(3)) among samples collected from the same run of the same type were high (0.73 to 0.93). Among samples collected from the same run but of different types, correlations were moderate to high (0.45 to 0.85); however, correlations were somewhat lower at the low tertile (-0.31 to 0.75). Estimated mean recovery efficiencies ranged from 0.22 to 0.25 CFU/particle with total CVs of approximately 84 to 97%. Estimated detection limits ranged from 35 to 39 particles/m(3). These results will enable investigators to conduct environmental sampling, quantify contamination levels, and conduct risk assessments of B. anthracis.
Keywords
Aerosol-sampling; Air-sampling; Air-sampling-techniques; Sampling-methods; Particle-aerodynamics; Particulate-sampling-methods; Airborne-particles; Bacteria; Microorganisms; Samplers; Filters; Membrane-filters; Author Keywords: aerosols; bioaerosols; emergency response; recovery efficiency; sampling methods
Contact
Cheryl Fairfield Estill, CDC, National Institute for Occupational Safety and Health, MS R-14, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
CODEN
JOEHA2
CAS No.
9002-84-0
Publication Date
20110301
Document Type
Journal Article
Fiscal Year
2011
NTIS Accession No.
NTIS Price
Issue of Publication
3
ISSN
1545-9624
NIOSH Division
DART; DSHEFS
Priority Area
Manufacturing; Services: Public Safety
Source Name
Journal of Occupational and Environmental Hygiene
State
OH; UT; GA; VA
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