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Quantitative plasma biomarker analysis in HDI exposure assessment.

Flack-SL; Fent-KW; Trelles Gaines-LG; Thomasen-JM; Wittaker-S; Ball-LM; Nylander-French-LA
Ann Occup Hyg 2010 Jan; 54(1):41-54
Quantification of amines in biological samples is important for evaluating occupational exposure to diisocyanates. In this study, we describe the quantification of 1,6-hexamethylene diamine (HDA) levels in hydrolyzed plasma of 46 spray painters applying 1,6-hexamethylene diisocyanate (HDI)-containing paint in vehicle repair shops collected during repeated visits to their workplace and their relationship with dermal and inhalation exposure to HDI monomer. HDA was detected in 76% of plasma samples, as heptafluorobutyryl derivatives, and the range of HDA concentrations was < or =0.02-0.92 microg l(-1). After log-transformation of the data, the correlation between plasma HDA levels and HDI inhalation exposure measured on the same workday was low (N = 108, r = 0.22, P = 0.026) compared with the correlation between plasma HDA levels and inhalation exposure occurring approximately 20 to 60 days before blood collection (N = 29, r = 0.57, P = 0.0014). The correlation between plasma HDA levels and HDI dermal exposure measured on the same workday, although statistically significant, was low (N = 108, r = 0.22, P = 0.040) while the correlation between HDA and dermal exposure occurring approximately 20 to 60 days before blood collection was slightly improved (N = 29, r = 0.36, P = 0.053). We evaluated various workplace factors and controls (i.e. location, personal protective equipment use and paint booth type) as modifiers of plasma HDA levels. Workers using a downdraft-ventilated booth had significantly lower plasma HDA levels relative to semi-downdraft and crossdraft booth types (P = 0.0108); this trend was comparable to HDI inhalation and dermal exposure levels stratified by booth type. These findings indicate that HDA concentration in hydrolyzed plasma may be used as a biomarker of cumulative inhalation and dermal exposure to HDI and for investigating the effectiveness of exposure controls in the workplace.
Airborne-particles; Biological-effects; Biological-systems; Biomarkers; Breathing; Breathing-zone; Exposure-assessment; Exposure-levels; Inhalants; Lung-irritants; Particle-aerodynamics; Particulates; Pulmonary-system; Quantitative-analysis; Respiratory-hypersensitivity; Respiratory-irritants; Statistical-analysis; Workplace-studies; Paint-spraying; Paint-shops; Painters; Spray-painting; Author Keywords: biomarker; dermal exposure; 1,6-hexamethylene diamine (HDA); 1,6-hexamethylene diisocyanate (HDI); inhalation exposure; plasma
Leena A. Nylander-French, Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, CB #7431, Rosenau Hall, Chapel Hill, NC 27599-7431
Publication Date
Document Type
Journal Article
Email Address
Funding Type
Fiscal Year
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-007598; Grant-Number-R01-OH-009086
Issue of Publication
Source Name
Annals of Occupational Hygiene
Performing Organization
University of North Carolina, Chapel Hill, North Carolina