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Development of a rapid ATP bioluminescence assay for biocidal susceptibility testing of rapidly growing mycobacteria.

Authors
Kapoor-R; Yadav-JS
Source
J Clin Microbiol 2010 Oct; 48(10):3725-3728
NIOSHTIC No.
20038182
Abstract
An ATP-based biocide susceptibility assay for mycobacteria was developed by optimizing the cell lysis and assay conditions. Compared to the conventional agar plating method, the assay was rapid (1.5 h) and showed high sensitivity and specificity as determined by receiver operating characteristic (ROC) analysis. The test species, Mycobacterium immunogenum, M. chelonae, and M. abscessus, showed various susceptibilities to the glutaraldehyde- and isothiazolone-based test biocides.
Keywords
Biocides; Adenosines; Bioassays; Mycology; Bacteria; Bacterial-cultures; Microbial-test-systems; Cytolysis; Disinfectants; Pharmacology; Humans; Luminescence; Sensitivity-testing; Thiazoles; Aldehydes
Contact
Jagjit S. Yadav, Department of Environmental Health, University of Cincinnati, College of Medicine, Cincinnati, OH 45267-0056
CODEN
JCMIDW
CAS No.
111-30-8; 55965-84-9
Publication Date
20101001
Document Type
Journal Article
Email Address
jagjit.yadav@uc.edu
Funding Type
Grant
Fiscal Year
2011
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-007364
Issue of Publication
10
ISSN
0095-1137
Source Name
Journal of Clinical Microbiology
State
OH
Performing Organization
University of Cincinnati
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