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A rapid HPLC analysis for dermal penetration: the case study of 4-chloro-3-methylphenol (CMP) from metal working fluid Trim VX.

Authors
Zang-LY; Barbero-AM; Frasch-HF
Source
Open Anal Chem J 2010 Mar; 4:10-17
NIOSHTIC No.
20037939
Abstract
A rapid and simple HPLC method has been developed for the determination of 4-chloro-3-methylphenol (CMP) in the metal working fluid (MWF), Trim VX and in experiments designed to measure the dermal penetration of CMP from MWF. The method used 2-methyl-4,6-dinitrophenol as an internal standard (IS). Water was used as the extraction solvent. The retention times of CMP and internal standard (IS) were 5.56 and 6.67 min, respectively. The standard plots of CMP are linear over the range of 62.5 - 2,500 ngmL-1 with correlation coefficients of 0.999. The limit of quantification (LOQ) for CMP was estimated to be 62.5 ngmL-1 and the limit of detection (LOD) was approached to be 20 ngmL-1. Reproducibility of the sample handling and HPLC analysis had a relative standard deviation (RSD) of less than 6%. The average recoveries for the analyte in Trim VX were better than 92%. Using the method, we found that Trim VX contains 2% CMP. In our preliminary dermal penetration study, the permeability coefficient kp and lag time (tau) were estimated to be 4.88..10-3 cmh-1 and 1.65 h, respectively.
Keywords
Liquid-chromatography; Analytical-methods; Metal-compounds; Metalworking; Metalworking-fluids; Skin-absorption; Skin-exposure; Skin-irritants; Analytical-processes; Author Keywords: CMP; MWFs; HPLC
Contact
H. Frederick Frasch, Exposure Assessment Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888, USA
CODEN
OACJAN
CAS No.
59-50-7
Publication Date
20100301
Document Type
Journal Article
Email Address
hbf9@cdc.gov
Fiscal Year
2010
NTIS Accession No.
NTIS Price
ISSN
1874-0650
NIOSH Division
HELD
Source Name
The Open Analytical Chemistry Journal
State
WV
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