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Target size of neurotoxic esterase and acetylcholinesterase as determined by radiation inactivation.

Authors
Carrington-CD; Fluke-DJ; Abou-Donia-MB
Source
Biochemical J 1985 Nov; 231(3):789-792
NIOSHTIC No.
20037451
Abstract
The target size of neurotoxic esterase (NTE), the putative target site for the initiation of organophosphorus-compound-induced delayed neurotoxicity, and acetylcholinesterase (AChE) from hen brain were examined by determining the rate at which the activities of the esterases were destroyed by ionizing irradiation. Samples of hen brain were prepared by slowly drying a microsomal preparation under vacuum. The dried samples were then irradiated with electrons from a 1 MeV Van de Graaff generator. The doses ranged from 0 to 28 Mrad. The radiation doses were calibrated by the rate of inactivation of T1-bacteriophage plaque induction. Following the irradiation procedure, the samples were resuspended in buffer and enzymic activity was measured. The target size of NTE from hen brain was determined to be about 105 kDa, whereas hen brain AChE was found to have a target size of about 53 kDa. The target size of NTE was found to be similar in experiments with rat brain and cat brain. In addition, commercial preparations of electric-eel electric-organ AChE and horse serum butyrylcholinesterase were found to have target sizes that were identical with each other, and also were very similar to that of AChE from hen brain.
Keywords
Neurotoxic-effects; Neurotoxicity; Exposure-assessment; Exposure-levels; Central-nervous-system; Laboratory-animals; Animal-studies; Brain-function; Ionizing-radiation; Irradiation
CODEN
BIJOAK
Publication Date
19851101
Document Type
Journal Article
Funding Amount
665055
Funding Type
Grant
Fiscal Year
1986
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-02003; Grant-Number-R01-OH-00823
Issue of Publication
3
ISSN
0264-6021
Priority Area
Neurotoxic-effects
Source Name
Biochemical Journal
State
NC
Performing Organization
Duke University, Durham, North Carolina
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