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Alcohol up-regulates TLR2 through a NO/cGMP dependent pathway.

Authors
Bailey-KL; Sisson-JH; Romberger-DJ; Robinson-JE; Wyatt-TA
Source
Alcohol Clin Exp Res 2010 Jan; 34(1):51-56
NIOSHTIC No.
20037336
Abstract
Background: Heavy alcohol consumption is associated with severe bronchitis. This is likely related to increased inflammation in the airways of alcohol abusers. Toll-like receptor 2 (TLR2) is an important mediator of inflammation in the airway epithelium. TLR2 initiates an inflammatory cascade in response to gram-positive bacteria. We have previously shown that alcohol up-regulates TLR2 in the airway epithelium. However, the mechanism of alcohol-mediated up-regulation of TLR2 has not been identified. Methods: A human airway epithelial cell line, 16HBE14o-, was exposed to biologically relevant concentrations of alcohol (100 mM) in the presence and absence of Nw-Nitro-L-arginine methyl ester hydrochloride, a nitric oxide (NO) synthase inhibitor; and Rp-8-Br-cGMP-S, an antagonist analogue of cGMP. TLR2 was measured using real-time PCR and Western blots. In addition, 16HBE14o- cells were incubated with sodium nitroprusside (SNP), an NO donor, and 8-Br-cGMP, a cGMP analogue. TLR2 was measured using real-time PCR. Results: Nw-Nitro-L-arginine methyl ester hydrochloride blocked the alcohol-mediated up-regulation of TLR2. This indicates that NO plays a key role in alcohol's up-regulation of TLR2. SNP, a NO donor, up-regulated TLR2. Rp-8-Br-CGMP-S attenuated alcohol's up-regulation of TLR2, suggesting that NO was working through cGMP/PKG. 8-Br-cGMP up-regulated TLR2, also demonstrating the importance of cGMP/PKG. Conclusions: Alcohol up-regulates TLR2 through a NO/cGMP/PKG dependent pathway in the airway epithelium. This is an important observation in the understanding how alcohol modulates airway inflammation. In addition, this is the first time that cyclic nucleotides have been shown to play a role in the regulation of TLR2.
Keywords
Alcohols; Alcoholism; Lung-disease; Lung-function; Respiratory-irritants; Respiratory-system-disorders; Biological-effects; Humans; Ethanols; Nucleotides; Author Keywords: Lung; Nitric Oxide; Cyclic Nucleotide; Ethanol; Inflammation
Contact
Kristina L. Bailey, MD, Pulmonary, Critical Care, Sleep & Allergy Medicine, Department of Internal Medicine, University of Nebraska Medical Center, Omaha, NE 68198-5915
CODEN
ACRSDM
CAS No.
10102-43-9
Publication Date
20100101
Document Type
Journal Article
Email Address
kbailey@unmc.edu
Funding Type
Grant
Fiscal Year
2010
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-008539
Issue of Publication
1
ISSN
0145-6008
Priority Area
Agriculture, Forestry and Fishing
Source Name
Alcoholism: Clinical and Experimental Research
State
NE
Performing Organization
University of Nebraska
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