Influence of particles on benzo(a)pyrene metabolism.
Warshawsky-D; Reilman-R; Cheu-J; Radike-M
Toxicologist 1992 Feb; 12(1):329
Epidemiological and experimental studies indicate that particles and chemical carcinogens are related to the development of respiratory disease. The long term objective is to investigate the role that pulmonary alveolar macrophages (AM) play in the particulate-dependent response of the lung to benzo(a)pyrene (BaP) via mechanisms involving BaP metabolism. An important biological response to inhaled particles is phagocytosis by AM and clearance from the lung. The comparative viability of the AM in the presence of ferric oxide, aluminum oxide, and crystalline and amorphous forms of silica was undertaken to determine the noncytotoxic doses during phagocytosis necessary for metabolic studies. AM from male-Syrian Golden hamsters, known to be susceptible to the formation of lung tumors by BaP-coated particles, were incubated with 0.0 to 0.5 mg of respirable size particles. After 48 hours the viability of AM for ferric oxide and aluminum oxide at 0.5 mg doses was similar to the controls. In the presence of crystalline and amorphous silica, the viability was similar to controls at 0.01 mg. At 0.05 mg silica the viability dropped to 40% and at 0.5 mg the viability was zero. These BaP-coated particles have a differential effect on the metabolism of BaP by the AM.
Carcinogens; Respiratory-system-disorders; Laboratory-animals; Lung-cancer; Lung-function; Lung-tissue; Lung-disease; Lung-disorders; Lung; Pulmonary-cancer; Pulmonary-disorders; Pulmonary-system-disorders; Particulates
The Toxicologist. Society of Toxicology 31st Annual Meeting, February 23-27,1992, Seattle, Washingtion
University of Cincinnati, Cincinnati, Ohio