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Production of hypersensitivity pneumonitis (HP) in mice by inhalation of spores of Micropolyspora faeni.

Authors
Kaliszewski-SD; Thorne-PS; Bleuer-SA
Source
Toxicologist 1992 Feb; 12(1):223
NIOSHTIC No.
20037082
Abstract
Farmers are exposed daily to an airborne mixture of respiratory irritants, microbial toxins, nuisance dust and a multitude of aeroallergens derived from plants, animals, arthropods, and microbes. Farmer's Lung disease (FLD) is a type of HP characterized by granulomatous interstitial lesions and lymphocytosis. In the midwest, FLD is nearly always linked to exposure to M. faeni (Faeni rectivirgula) , found in "moldy" hay. A murine model was developed using inhalation exposure only, to allow study of the immunologic processes accompanying FLD. M. faeni was cultured at 50C, then washed, concentrated, and homogenized. Aerosols of this preparation were generated into exposure chambers using a PITT#1 nebulizer, yielding an aerosol with a mass median diameter of 1.2 micro (Sigma(g)=3.25) and a mean count diameter of 0.90 micro. At the highest dose, the M. faeni exposure system produced concentrations of 5.2 1.5 mg/m3 and 1.7 x 10(9) spores/m3. Viable counts exceeded our detection limit: 1 x 10(7) CFU/m3. Lower exposure levels were obtained by dilution. Groups of 10 mice inhaled M. faeni aerosols for 30 min each exposure either once each wk for 4 or 8 wk, 5 day/wk for 1 or 2 wk, 5 days/wk for 2 wk plus a Day 16 challenge, or were sham exposed. Histopathological examination of lung sections demonstrated marked perivascular and peribronchiolar histiocytosis, localized acute inflammatory cells, fibrosis, and giant cells in granulomatous lesions in mice from several exposure protocols but not in any of the sham exposed mice. This study demonstrates that histopathology characteristic of FLD can be induced using an adjuvantfree inhalation model.
Keywords
Animal-studies; Animals; Inhalation-studies; Inhalants; Airborne-dusts; Airborne-particles; Lung-disease; Lung-function; Lung-irritants; Lung-disease; Aerosol-particles; Aerosols; Exposure-assessment; Exposure-levels; Exposure-limits; Exposure-methods; Cell-function; Fibrosis; Respiratory-system-disorders; Pulmonary-system-disorders
Publication Date
19920201
Document Type
Abstract
Funding Amount
1020020
Funding Type
Cooperative Agreement
Fiscal Year
1992
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U07-CCU-706145
Issue of Publication
1
ISSN
0731-9193
Source Name
The Toxicologist. Society of Toxicology 31st Annual Meeting, February 23-27,1992, Seattle, Washingtion
State
IA; OH
Performing Organization
University of Iowa, Iowa City, Iowa
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