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Inertial microfluidics for sheath-less high-throughput flow cytometry.

Authors
Bhagat-AA; Kuntaegowdanahalli-SS; Kaval-N; Seliskar-CJ; Papautsky-I
Source
Biomed Microdevices 2010 Apr; 12(2):187-195
NIOSHTIC No.
20037015
Abstract
Flow cytometer is a powerful single cell analysis tool that allows multi-parametric study of suspended cells. Most commercial flow cytometers available today are bulky, expensive instruments requiring high maintenance costs and specially trained personnel for operation. Hence, there is a need to develop a low cost, portable alternative that will aid in making this powerful research tool more accessible. In this paper we describe a sheath-less, on-chip flow cytometry system based on the principle of Dean coupled inertial microfluidics. The design takes advantage of the Dean drag and inertial lift forces acting on particles flowing through a spiral microchannel to focus them in 3-D at a single position across the microchannel cross-section. Unlike the previously reported micro-flow cytometers, the developed system relies entirely on the microchannel geometry for particle focusing, eliminating the need for complex microchannel designs and additional microfluidic plumbing associated with sheath-based techniques. In this work, a 10-loop spiral microchannel 100 Ám wide and 50 Ám high was used to focus 6 Ám particles in 3-D. The focused particle stream was detected with a laser induced fluorescence (LIF) setup. The microfluidic system was shown to have a high throughput of 2,100 particles/sec. Finally, the viability of the developed technique for cell counting was demonstrated using SH-SY5Y neuroblastoma cells. The passive focusing principle and the planar nature of the described design will permit easy integration with existing lab-on-a-chip (LOC) systems.
Keywords
Fluid-mechanics; Fluids; Particulates; Particle-aerodynamics; Cell-function; Cellular-function; Laboratory-equipment; Laboratory-testing; Author Keywords: Microfluidics; Flow cytometry; Cell counting
Contact
Ian Papautsky, Department of Electrical and Computer Engineering, University of Cincinnati, 814 Rhodes Hall, ML030, Cincinnati, OH 45221, USA
CODEN
BMICFC
Publication Date
20100401
Document Type
Journal Article
Email Address
ian.papautsky@uc.edu
Funding Type
Grant
Fiscal Year
2010
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-T42-OH-008432
Issue of Publication
2
ISSN
1387-2176
Source Name
Biomedical Microdevices
State
OH
Performing Organization
University of Cincinnati
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