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In vitro lymphocyte responses in contact hypersensitivity III.

Authors
Milner-JE
Source
J Invest Dermatol 1972 Jun; 58(6):388-391
NIOSHTIC No.
20036830
Abstract
Guinea pigs were sensitized to one of two contact allergens: dinitrofluorobenzene (DNFH) or paraphenylenediamine (PDA). Sensitization was accomplished by footpad injections of the hapten conjugated to an extract of soluble guinea pig epidermal proteins in Freund's complete adjuvant. Lymphocytes from animals with contact hypersensitivity to DNFB or PDA were cultured in vitro with guinea pig epidermal proteins conjugated to DNFB or PDA. Responses to these antigens, as measured by incorporation of tritiated thymidine into DNA, indicated that lymphocyte transformation in vitro can distinguish between sensitivity to either of these allergens.
Keywords
Allergic-dermatitis; Allergic-disorders; Allergic-reactions; Allergies; Antigens; Dermatitis; Dermatosis; Exposure-assessment; Exposure-levels; Exposure-methods; Hypersensitivity; Immunology; Laboratory-animals; Laboratory-testing; Skin-disorders; Skin-exposure; Skin-irritants; Skin-sensitivity
Contact
John E Milner, Department of Medicine, School of Medicine, and the Department of Environmental Health, School of Public Health, University of Washington, Seattle, Washington, 98195
CODEN
JIDEAE
Publication Date
19720601
Document Type
Journal Article
Funding Amount
140693
Funding Type
Grant
Fiscal Year
1972
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-00321
Issue of Publication
6
ISSN
0022-202X
Priority Area
Dermatitis
Source Name
Journal of Investigative Dermatology
State
WA
Performing Organization
University of Washington, Seattle, Washington
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