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Carbon nanotubes degraded by neutrophil myeloperoxidase induce less pulmonary inflammation.

Authors
Kagan-VE; Konduru-NV; Feng-W; Allen-BL; Conroy-J; Volkov-Y; Vlasova-II; Belikova-NA; Yanamala-N; Kapralov-A; Tyurina-YY; Shi-J; Kisin-ER; Murray-AR; Franks-J; Stolz-D; Gou-P; Klein-Seetharaman-J; Fadeel-B; Star-A; Shvedova-AA
Source
Nat Nanotechnol 2010 May; 5(5):354-359
NIOSHTIC No.
20036720
Abstract
We have shown previously that single-walled carbon nanotubes can be catalytically biodegraded over several weeks by the plant-derived enzyme, horseradish peroxidase1. However, whether peroxidase intermediates generated inside human cells or biofluids are involved in the biodegradation of carbon nanotubes has not been explored. Here, we show that hypochlorite and reactive radical intermediates of the human neutrophil enzyme myeloperoxidase catalyse the biodegradation of single-walled carbon nanotubes in vitro, in neutrophils and to a lesser degree in macrophages. Molecular modelling suggests that interactions of basic amino acids of the enzyme with the carboxyls on the carbon nanotubes position the nanotubes near the catalytic site. Importantly, the biodegraded nanotubes do not generate an inflammatory response when aspirated into the lungs of mice. Our findings suggest that the extent to which carbon nanotubes are biodegraded may be a major determinant of the scale and severity of the associated inflammatory responses in exposed individuals.
Keywords
Biodegradation; Biological-factors; Cell-function; Cellular-reactions; Dust-exposure; Exposure-assessment; Exposure-levels; Exposure-methods; Inhalation-studies; Laboratory-animals; Laboratory-testing; Lung-disorders; Lung-irritants; Molecular-biology; Particulates; Peroxidases; Physiological-effects; Pulmonary-disorders; Pulmonary-system; Respiratory-irritants
Publication Date
20100501
Document Type
Journal Article
Funding Type
Grant
Fiscal Year
2010
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-008282
Issue of Publication
5
ISSN
1748-3387
NIOSH Division
HELD
Source Name
Nature Nanotechnology
State
WV
Performing Organization
University of Pittsburgh at Pittsburgh
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