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Direct fibrogenic effects of dispersed single-walled carbon nanotubes on human lung fibroblasts.

Authors
Wang-LY; Mercer-RR; Rojanasakul-Y; Qiu-AJ; Lu-YJ; Scabilloni-JF; Wu-NQ; Castranova-V
Source
J Toxicol Environ Health, A 2010 Jan; 73(5-6):410-422
NIOSHTIC No.
20036495
Abstract
Nanomaterials, including single-walled carbon nanotubes (SWCNT), are being developed for a variety of commercial products. However, adverse health effects attributed to these new materials are not well understood. Recent reports showed that exposure of mice to dispersed SWCNT (DSWCNT) produced a rapid and progressive interstitial lung fibrosis without persistent inflammation. To understand the mechanism underlying this unusual fibrogenicity of DSWCNT, the present investigation focused on the direct bioactivity of DSWCNT using a cell culture of lung fibroblasts that represent a major cell type resident in the lung interstitium and responsible for the production of collagen matrix. At concentrations relevant to those used in vivo, in vitro exposure of lung fibroblasts to DSWCNT stimulated cell proliferation and induced collagen production without producing cell damage. One of the major matrix metalloproteinases (MMP), MMP-9, which is known to be involved in lung fibrosis, was also elevated by DSWCNT treatment both in vitro and in vivo. Taken together, these results suggest that direct stimulation of fibroblasts by DSWCNT translocated into the interstitium may play a significant role in DSWCNT-induced lung fibrosis. Our data also suggest that the dispersion status and/or size of the SWCNT structures is a critical factor in determining nanoparticle fibrogenicity and that MMP-9 may be involved in the fibrogenic process. The results obtained may aid in the development of in vitro models for rapid screening of the potential fibrogenicity of carbon nanotubes, which are lacking and urgently needed.
Keywords
Bioactivation; Biological-effects; Biomedical-engineering; Dust-exposure; Dust-particles; Exposure-assessment; Exposure-levels; Exposure-methods; Fibrous-dusts; Laboratory-animals; Laboratory-testing; Lung-disorders; Lung-fibrosis; Lung-function; Nanotechnology; Particulate-dust; Particulates; Physiological-effects; Pulmonary-system; Risk-factors; Toxic-effects
Contact
Liying Wang, CDC, NIOSH, HELD, 1095 Willowdale Rd, Morgantown, WV 26505
CODEN
JTEHD6
Publication Date
20100101
Document Type
Journal Article
Email Address
lmw6@cdc.gov
Fiscal Year
2010
NTIS Accession No.
NTIS Price
Issue of Publication
5-6
ISSN
1528-7394
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
Journal of Toxicology and Environmental Health, Part A: Current Issues
State
WV
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