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Analysis of free-radical scavenging of Yerba Mate (llex paraguriensis) using electron spin resonance and radical-induced DNA damage.

Authors
Leonard-SS; Hogans-VJ; Coppes-Petricorena-Z; Peer-CJ; Vining-TA; Fleming-DW; Harris-GK
Source
Food Chem 2010 Jan; 75(1):C14-C20
NIOSHTIC No.
20036352
Abstract
Mate (MT) is a popular South American beverage that has been used as a traditional medicine for centuries, spurring recent interest in its nutraceutical properties. MT is prepared as an infusion of leaves fromthe Yerba Mate (llex paraguriensis) tree. MT has been reported to have antioxidant properties in vitro and in vivo, but these have not been fully characterized in terms of effects against specific radicals. Accordingly, we examined the antioxidant effects of an MT infusion against hydroxyl and superoxide radicals in both chemical and cell culture assays. MT infusions were prepared at 3.10 g/L in boiling water and diluted to experimental dilutions from this stock. Electron spin resonance (ESR) experiments indicated that MT scavenged hydroxyl radicals (produced via the Fenton reaction) and superoxide radicals (produced via the xanthine/xanthine oxidase enzymatic reaction) at all concentrations tested (P <0.05). Further controls indicated that superoxide radical scavengingwas not due to xanthine oxidase inhibition. MT scavenged hydroxyl radicals and decreased cellular oxygen consumption in a dose-dependent manner in Cr(VI)-stimulated RAW 264.7 cells, based on ESR and oxygraph measurements (P < 0.05). Similarly, MT also inhibited hydroxyl-radical-induced lipid peroxidation and DNA damage in a dose-dependent manner in RAW 264.7 cells, based on malondialdehyde and Comet assay data (P < 0.05). This study indicates that MT possesses potent antioxidant effects against hydroxyl and superoxide radicals in both chemical and cell culture systems, as well as DNA-protective properties. These data further clarify the reported antioxidant effects of Yerba Mate infusions.
Keywords
Bioassays; Cell-biology; Cell-cultures; Cell-function; Chemical-analysis; Natural-products; Oxidation; Oxidative-processes; Pharmacodynamics; Statistical-analysis; Author Keywords: antioxidants; DNA damage; free radicals; lipid peroxidation; Yerba Mate
Contact
Stephen S. Leonard, CDC, NIOSH, Health Effects Laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505
CODEN
FOCHDJ
Publication Date
20101001
Document Type
Journal Article
Email Address
sel5@cdc.gov
Fiscal Year
2011
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
0308-8146
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
Food Chemistry
State
WV
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