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Serum cholinesterase inhibition in relation to paraoxonase-1 (PON1) status among organophosphate-exposed agricultural pesticide handlers.

Authors
Hofmann-JN; Keifer-MC; Furlong-CE; DeRoos-AJ; Farin-FM; Fenske-RA; vanBelle-G; Checkoway-H
Source
Environ Health Perspect 2009 Sep; 117(9):1402-1408
NIOSHTIC No.
20035897
Abstract
BACKGROUND: Animal studies have demonstrated that low paraoxonase-1 (PON1) status (i.e., low catalytic efficiency and/or low plasma PON1 activity) is associated with neurotoxic effects after exposure to several organophosphate (OP) insecticides. However, few human studies have investigated associations between PON1 status and intermediate end points, such as serum cholinesterase [butyrylcholinesterase (BuChE)] inhibition, among OP-exposed individuals. OBJECTIVES: We evaluated the relation between plasma PON1 status and BuChE inhibition among OP-exposed agricultural pesticide handlers. METHODS: Agricultural pesticide handlers in Washington State were recruited during the 2006 and 2007 spray seasons when they were seen for follow-up ChE testing by collaborating medical providers as part of a statewide monitoring program. Blood samples were collected from 163 participants and tested for PON1 status based on plasma PON1 activity [arylesterase (AREase)] and PON1 Q192R genotype. We evaluated percent change in BuChE activity from baseline level in relation to PON1 status. RESULTS: We observed significantly greater BuChE inhibition among QQ homozygotes relative to RR homozygotes (p = 0.036). Lower levels of plasma PON1 activity were significantly associated with greater BuChE inhibition (p = 0.004). These associations remained after adjustment for year, days since baseline test, age, and OP exposure in the last 30 days. CONCLUSIONS: We found that both low PON1 catalytic efficiency (i.e., the Q192 alloform) and low plasma PON1 activity were associated with BuChE inhibition among OP-exposed agricultural pesticide handlers. Corroborative findings from future studies with prospective collection of blood samples for PON1 testing, more sensitive markers of OP-related effects, and larger sample sizes are needed.
Keywords
Agricultural-industry; Agricultural-workers; Agriculture; Analytical-methods; Biochemical-analysis; Biochemical-tests; Biological-effects; Biological-monitoring; Biological-systems; Blood-analysis; Blood-serum; Chemical-deposition; Chemical-hypersensitivity; Exposure-levels; Exposure-methods; Humans; Insecticides; Occupational-exposure; Occupational-hazards; Pesticides; Pesticides-and-agricultural-chemicals; Statistical-analysis; Work-environment; Worker-health; Work-performance; Author Keywords: agriculture; cholinesterase; farmworkers; gene-environment interaction' organophosphates' paraoxonase; pesticides
Contact
J.N. Hofmann, Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Blvd., EPS 8109, MSC 7240, Bethesda, MD 20892-724
CODEN
EVHPAZ
Publication Date
20090901
Document Type
Journal Article
Email Address
hofmannjn@mail.nih.gov
Funding Type
Grant; Cooperative Agreement
Fiscal Year
2009
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-T42-OH-008433; Cooperative-Agreement-Number-U50-OH-007544
Issue of Publication
9
ISSN
0091-6765
Priority Area
Agriculture, Forestry and Fishing
Source Name
Environmental Health Perspectives
State
WA; MD
Performing Organization
University of Washington
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