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Exposure to crystalline silica or treatment with chlorphentermine increases alveolar-lavage vitamin-E levels.

Authors
Miles-PR; Bowman-L; Reasor-MJ
Source
FASEB J 1995 Mar; 9(4)(II):A717
NIOSHTIC No.
20035860
Abstract
Vitamin E may be an integral part of lung surfactant (Am. J. Physiol. 265:L133-L139, 1993). Therefore, we measured vitamin E levels in alveolar lavage materials from rats exposed to silica or treated with chlorphentermine (CP), two treatments which are known to increase surfactant phospholipids (PL) by different mechanisms. Silica exposure leads to increased PL synthesis, and CP treatment causes reduced PL degradation. HCI-washed and unwashed silica were used because exposure to each of them leads to different degrees of phospholipidosis. Exposure to HCI-washed silica results in a 17-fold increase in lavage PL and protein levels and a 12.2-fold increase in the amount of vitamin E. Exposure to unwashed silica leads to a 7-fold increase in PL and proteins and a 5.8-fold increase in lavage vitamin E. Following treatment of rats with CP, there are 15- to 19-fold increases in lavage PL and proteins and a 13.6-fold increase in vitamin E. Because surfactant synthesis occurs in the endoplasmic reticulum, we also measured vitamin E in lung microsomes. Both silica exposure and CP treatment lead to 1.8- to 2.5-fold increases in the lung microsomal levels of vitamin E. These results demonstrate that alveolar lavage vitamin E levels are elevated along with lavage PL and proteins and lung microsomal vitamin E levels are increased following exposure of rats to silica or treatment of the animals with CP.
Keywords
Biochemical-analysis; Biochemistry; Biological-effects; Biological-systems; Cell-biology; Cell-function; Cellular-reactions; Exposure-assessment; Exposure-methods; Inhalation-studies; Laboratory-animals; Laboratory-techniques; Laboratory-testing; Lung-function; Lung-irritants; Molecular-biology; Molecular-structure; Pulmonary-system; Silicates; Silicon-compounds
CODEN
FAJOEC
CAS No.
7631-86-9
Publication Date
19950310
Document Type
Abstract; Conference/Symposia Proceedings
Fiscal Year
1995
NTIS Accession No.
NTIS Price
Issue of Publication
4
ISSN
0892-6638
NIOSH Division
DRDS
Source Name
The FASEB Journal. Experimental Biology 95 - Annual Meeting of Professional Research Scientists, Atlanta, Georgia, April 9-13, 1995
State
WV
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