Mass-spectrometric analysis of hydroperoxy- and hydroxy-derivatives of cardiolipin and phosphatidylserine in cells and tissues induced by pro-apoptotic and pro-inflammatory stimuli.
Tyurin-VA; Tyurina-Y; Jung-MY; Tungekar-MA; Wasserloos-KJ; Bayir-H; Greenberger-JS; Kochanek-PM; Shvedova-AA; Pitt-B; Kagan-VE
J Chromatogr B 2009 Sep; 877(26):2863-2872
Oxidation of two anionic phospholipids - cardiolipin (CL) in mitochondria and phosphatidylserine (PS) in extramitochondrial compartments - is important signaling event, particularly during the execution of programmed cell death and clearance of apoptotic cells. Quantitative analysis of CL and PS oxidation products is central to understanding their molecular mechanisms of action. We combined the identification of diverse phospholipid molecular species by ESI-MS with quantitative assessments of lipid hydroperoxides using a fluorescence HPLC-based protocol. We characterized CL and PS oxidation products formed in a model system (cyt c/H2O2), in apoptotic cells (neurons, pulmonary artery endothelial cells) and mouse lung under inflammatory/oxidative stress conditions (hyperoxia, inhalation of single walled carbon nanotubes). Our results demonstrate the usefulness of this approach for quantitative assessments, identification of individual molecular species and structural characterization of anionic phospholipids that are involved in oxidative modification in cells and tissues.
Animals; Biochemistry; Biological-effects; Biological-factors; Biological-monitoring; Cell-biology; Metabolic-study; Metabolites; Molecular-biology; Neurological-reactions; Oxidation; Oxidative-metabolism; Quantitative-analysis; Work-areas; Work-environment; Worker-health; Workplace-studies; Nanotechnology
Vladimir A. Tyurin, Center for Free Radical and Antioxidant Health, University of Pittsburgh, 100 Technol Dr, Suite 323, Pittsburgh, PA 15219
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
University of Pittsburgh at Pittsburgh