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Quantitative identification of atrazine and its chlorinated metabolites in plasma.

Authors
Brzezicki-JM; Andersen-ME; Cranmer-BK; Tessari-JD
Source
J Anal Toxicol 2003 Nov-Dec; 27(8):569-573
NIOSHTIC No.
20035768
Abstract
The objective of this study was to develop an analytical method to detect and quantitate the chlorotriazine herbicide atrazine (ATRA), and its chlorinated metabolites [desethylatrazine (DE-ATRA), desisopropylatrazine (DI-ATRA), and diaminochlorotriazine (DACT)] in plasma. Control plasma separated from whole rat blood was fortified with known concentrations of ATRA, DE-ATRA, DI-ATRA, and DACT. These compounds were extracted from the plasma using a liquid-liquid extraction technique, and the resulting extracts were derivatized with tetrabutyl ammonium hydroxide and methyl iodide to produce methylated derivatives of ATRA and its chlorinated metabolites. Derivatized samples and standards were analyzed using gas chromatography-mass spectrometry with selected ion monitoring. Recoveries of fortified plasma samples ranged from 84% to 97% and were validated to 100 ng/mL. This analytical method was subsequently verified in a small-scale animal study to determine time course concentrations of chlorotriazines in plasma following a single oral gavage dose of ATRA to female Sprague Dawley rats.
Keywords
Chemical-composition; Chemical-hypersensitivity; Chemical-indicators; Chemical-properties; Chemical-reactions; Chemical-structure; Chemical-synthesis; Animal-studies; Animals; Blood-analysis; Blood-plasma; Blood-samples; Blood-sampling; Blood-serum; Blood-tests; Gas-chromatography; Gas-detectors; Gas-liquid-chromatography; Mass-spectrometry; Risk-analysis; Risk-factors; Exposure-levels; Exposure-limits; Exposure-methods; Gene-mutation; Genes; Genetic-disorders; Genetic-factors; Genetics; Genotoxic-effects; Genotoxicity; Cancer; Cancer-rates
Contact
J. M. Brzezicki, Department of Environmental and Radiological Health Sciences, Colorado State University, Ft Collins, Colorado 80523
CODEN
JATOD3
CAS No.
1912-24-9
Publication Date
20031101
Document Type
Journal Article
Email Address
jbrz@colostate.edu
Funding Type
Cooperative Agreement
Fiscal Year
2004
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U50-OH-007545
Issue of Publication
8
ISSN
0146-4760
Source Name
Journal of Analytical Toxicology
State
CO
Performing Organization
Colorado State University - Fort Collins
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