Metabolism and DNA binding of BaP-coated particles by hamster pulmonary alveolar macrophage.
Warshawsky-D; Reilman-R; Collins-T; Jaeger-M; Cheu-J; Radike-M; Talaska-G
Toxicologist 1994 Mar; 14(1):339
Epidemiological and experimental studies indicate that particles and chemical carcinogens are related to the development of respiratory disease. The long term objective is to investigate the role that pulmonary alveolar macrophages (AM) play in the particulate-dependent response of the lung to benzo(a)pyrene (BaP) metabolism. The comparative metabolism and DNA binding of SaP-coated particles by AM using particles, ferric oxide and crystalline and amorphous silica, was undertaken to determine the differential metabolism of BaP. AM from male-Syrian Golden hamsters were incubated with BaP-coated respirable size particles at a dose of 3 ug of BaP and 50 ug particle. After 24 hours the metabolic products in the extractable fraction were greater for BaP-coated particles than BaP alone with the dihydrodiols of BaP being the major metabolites. Of the dihydrodiols present, the 7,8-dihydrodiol was significantly greater for the BaP-coated particles than BaP alone. This was associated with greater level of binding of the BaP-DNA adduct #2 relative to BaP-DNA adduct #1, the (+) antidiol epoxide of BaP using 32P-postlabeling. The data indicate that these BaP-coated particles have a differential effect on the metabolism of BaP by the AM.
Laboratory-testing; Toxins; Toxicology; Toxic-effects; Exposure-assessment; Exposure-levels; Laboratory-animals; Animal-studies; Iron-oxides; Pyrenes; DNA-adducts; Mineral-dusts; Quartz-dust; Alveolar-cells
1345-25-1; 50-32-8; 14808-60-7
The Toxicologist. Society of Toxicology 33rd Annual Meeting, March 13-17, 1994, Dallas, Texas
Univesity of Cincinnati, Cincinnati, Ohio