Comparative developmental toxicity of urethane (URE), 2-methoxyethanol (2ME) and methotrexate (MTX) in Drosophila melanogaster obtained from two sources.
Toxicologist 1994 Mar; 14(1):166
The Drosophila Bioassay developed at NIOSH has been proposed as a screening test to help establish priorities for assessment of developmental toxicity in vivo (Lynch et al., Teratogenesis Carcinog. Mutagen. 11: 147-173, 1991). An independent laboratory used Oregon-R wild-type fruit flies obtained from Carolina Biological Supply Co. (CBSC) in this published effort. In order to further characterize the bioassay, an in-house study was performed 1) to determine if flies obtained from different sources respond both qualitatively and quantitatively in the same way to selected known developmental toxicants and 2) to compare the reproducibility of bioassay data obtained at different times in different labs using the same chemicals. Concurrent bioassays using flies obtained from both CBSC and the Mid-American Drosophila Stock Center were conducted at 25°C. Three developmental toxicants URE (5 and 7.5 mg/vial), 2ME (10 and 15 mg/vial) and MTX (100 and 200 microg/vial) were evaluated in separate experiments. Both URE and 2ME, previously documented to increase the incidence of bent bristles in developing Drosophila, statistically increased the incidence of this defect at both concentrations and in both strains1compared to concurrent controls. MTX, reported to increase the incidence of wing notches, statistically increased the incidence of this wing blade defect in both strains and at both concentrations. These data indicate that the Drosophila Bioassay is, not dependent on fly source and that bioassay results (at least with these three known developmental toxicants) can be replicated in independent labs. These results support further utilization of this bioassay as a screening test in developmental toxicology studies.
Bioassays; Laboratory-testing; Toxins; Toxicology; Toxic-effects
The Toxicologist. Society of Toxicology 33rd Annual Meeting, March 13-17, 1994, Dallas, Texas