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Evaluation of irritancy and sensitization potential of metalworking fluid mixtures and components.

Authors
Anderson-SE; Brown-KK; Butterworth-LF; Fedorowicz-A; Jackson-LG; Frasch-HF; Beezhold-D; Munson-AE; Meade-BJ
Source
J Immunotoxicol 2009 Mar; 6(1):19-29
NIOSHTIC No.
20035389
Abstract
There are approximately 1.2 million workers exposed to metalworking fluids (MWF), which are used to reduce the heat and friction associated with industrial machining and grinding operations. Irritancy and sensitization potential of 9 National Toxicology Program (NTP) nominated MWFs (TRIM 229, TRIM VX, TRIM SC210, CIMTECH 310, CIMPERIAL 1070, CIMSTAR 3800, SYNTILO 1023, SUPEREDGE 6768, and CLEAREDGE 6584) were examined in a combined local lymph node assay (LLNA). BALB/c mice were dermally exposed to each MWF at concentrations up to 50%. Significant irritation was observed after dermal exposure to all MWFs except CIMTECH 310 and SYNTILO 1023. Of the 9 MWFs, 6 induced greater than a 3-fold increase in lymphocyte proliferation and 7 tested positive in the irritancy assay. TRIM VX yielded the lowest EC3 value (6.9%) with respect to lymphocyte proliferation. Chemical components of TRIM VX identified using HPLC were screened for sensitization potential using structural activity relationship (SAR) modeling and the LLNA. TOPKAT predicted triethanolamine (TEA) as a sensitizer while Derek for Windows predicted only 4-chloro-3-methylphenol (CMP) to be positive for sensitization. When tested in the LLNA only CMP (EC3 = 11.6%) and oleic acid (OA) (EC3 = 29.7%) were identified as sensitizers. Exposure to all tested TRIM VX components resulted in statistically significant irritation. An additive proliferative response was observed when mixtures of the two identified sensitizing TRIM VX components, OA and CMP, were tested in the LLNA. This is one explanation of why the EC3 value of TRIM VX, with respect to lymphocyte proliferation, is lower than those assigned to its sensitizing components.
Keywords
Metalworking-fluids; Metalworking-industry; Metalworking; Skin-disorders; Skin-exposure; Skin-irritants; Dermatitis; Dermatosis; Contact-dermatitis; Author Keywords: metalworking fluids; LLNA; contact dermatitis; irritancy
Contact
Stacey E. Anderson, Ph.D., National Institute for Occupational Safety and Health (NIOSH), 1095 Willowdale Drive, Morgantown, WV 26505, USA
CODEN
JIOMAP
Publication Date
20090301
Document Type
Journal Article
Email Address
sanderson4@cdc.gov
Fiscal Year
2009
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
1547-691X
NIOSH Division
HELD; DART
Priority Area
Construction; Manufacturing
Source Name
Journal of Immunotoxicology
State
WV
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