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A rapid most-probable-number-based enzyme-linked immunosorbent assay for the detection and enumeration of Salmonella Typhimurium in poultry wastewater.

Authors
Goodridge-CF; Goodridge-LD; Gottfried-D; Wyvill-JC
Source
J Food Prot 2003 Dec; 66(12):2302-2306
NIOSHTIC No.
20035330
Abstract
The rapid and accurate detection and enumeration of low levels of Salmonella Typhimurium in food processing facilities are critical components of an effective hazard analysis critical control point program. The objective of this study was to develop a rapid (8 h) most probable number (MPN)-enzyme-linked immunosorbent assay (ELISA) for the detection and enumeration of Salmonella Typhimurium in wastewater. The specific objectives were to (i) characterize poly- and monoclonal Salmonella Typhimurium-specific antibodies in order to select the most specific and sensitive antibody for Salmonella Typhimurium detection, and (ii) validate the MPN assay through a correlation between the 8-h MPN-ELISA and the traditional 48-h Salmonella Typhimurium MPN method in poultry scald water. Poultry scald water samples were spiked with 10 and 50 CFU/ml of Salmonella Typhimurium. The traditional MPN method used a 48-h enrichment period followed by an analysis, while the MPN-ELISA used a 5-h enrichment period followed by a 3-h ELISA analysis. No differences (P < 0.05) were found between the traditional MPN and the MPN-ELISA, indicating the promise of the MPN-ELISA for the rapid detection and enumeration of Salmonella Typhimurium within an 8-h shift. This abbreviated assay will permit increased product sampling and more rapid movement of food between production and processing, resulting in reduced spoilage and quality losses.
Keywords
Ecological-systems; Education; Environmental-contamination; Environmental-exposure; Environmental-factors; Environmental-hazards; Environmental-health; Environmental-pollution; Environmental-protection; Environmental-stress; Contaminated-food; Food; Food-contaminants; Food-handlers; Food-processing; Food-processing-equipment; Food-processing-industry; Food-processing-workers; Food-services; Food-spoilage; Factory-workers; Work-environment; Work-practices; Worker-health; Workers; Workplace-monitoring; Workplace-studies; Health-hazards; Health-standards; Safety-climate; Safety-education; Safety-equipment; Safety-measures; Safety-monitoring; Safety-personnel; Safety-practices; Safety-programs; Bacteria; Bacterial-cultures; Bacterial-disease; Bacterial-infections; Bactericides; Bacteriology; Risk-analysis; Risk-factors; Samplers; Sampling; Pathogenesis; Pathogenicity; Pathogens; Slaughterhouses; Microbial-test-systems; Microbiology; Microchemistry; Microorganisms; Microscopic-analysis; Microsomal-enzymes
Contact
C. Goodridge, Electro-Optics, Environment and Materials Laboratory, Georgia Tech Research Institute, Atlanta, Georgia 30332-0823
CODEN
JFPRDR
Publication Date
20031201
Document Type
Journal Article
Email Address
cgoodrid@uwyo.edu
Funding Type
Agriculture; Cooperative Agreement
Fiscal Year
2004
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U50-OH-007545
Issue of Publication
12
ISSN
0362-028X
Source Name
Journal of Food Protection
State
GA; CO
Performing Organization
Colorado State University - Fort Collins
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