Real-time measurement of fluorescence spectra from single airborne biological particles.
Hill-SC; Pinnick-RG; Niles-S; Pan-YL; Holler-S; Chang-RK; Bottiger-J; Chen-BT; Orr-CS; Feather-G
Field Anal Chem Tech 1999 Apr; 3(4-5):221-239
Improved real-time methods for characterizing airborne biological particles are needed. Here we review our efforts in developing techniques for measuring the laser-induced fluorescence (total and spectrally dispersed) of individual airborne particles, and describe our present system, which can measure fluorescence spectra of single micrometer-sized bioaerosol particles with good signal-to-noise ratios. We demonstrate the capability of this system by showing measured spectra of a variety of airborne particles generated in the laboratory from road dust, ammonium sulfate, Bacillus subtilis and other bacteria prepared under various conditions, allergens, cigarette smoke, and chicken-house dust. These spectra illustrate the capability of the system to distinguish between some biological and nonbiological aerosols, and among several types of laboratory-generated biological aerosols. We suggest improvements needed to make our system field portable.
Analytical-processes; Analytical-instruments; Airborne-particles; Biological-material; Aerosols; Aerosol-particles;
Author Keywords: bioaerosols; aerosol detection; fluorescence spectroscopy; instrumentation; single-particle fluorescence; biological aerosols
Field Analytical Chemistry and Technology