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Diesel exhaust particulate material expression of in vitro genotoxic activities when dispersed into a phospholipid component of lung surfactant.

Authors
Shi-XC; Keane-MJ; Ong-TM; Harrison-JC; Slaven-JE; Bugarski-AD; Gautam-M; Wallace-WE
Source
J Phys: Conf Ser, Inhaled Particles X 2009 Mar; 151(1):012021
NIOSHTIC No.
20035189
Abstract
Bacterial mutagenicity and mammalian cell chromosomal and DNA damage in vitro assays were performed on a diesel exhaust particulate material (DPM) standard in two preparations: as an organic solvent extract, and as an aqueous dispersion in a simulated pulmonary surfactant. U.S. National Institute for Standards and Technology DPM SRM 2975 expressed mutagenic activity in the Salmonella reversion assay, and for in vitro genotoxicity to mammalian cells as micronucleus induction and as DNA damage in both preparations: as an acetone extract of the DPM mixed into dimethylsulfoxide, and as a mixture of whole DPM in a dispersion of dipalmitoyl phosphatidyl choline. Dispersion in surfactant was used to model the conditioning of DPM depositing on the deep respiratory airways of the lung. DPM solid residue after acetone extraction was inactive when assayed as a surfactant dispersion in the micronucleus induction assay, as was surfactant dispersion of a respirable particulate carbon black. In general, a given mass of the DPM in surfactant dispersion expressed greater activity than the solvent extract of an equal mass of DPM.
Keywords
Bacteria; Bacterial-disease; Bacterial-dusts; Biohazards; Biological-effects; Biological-factors; Chemical-properties; Chemical-reactions; Chromosome-disorders; Cell-biology; Cell-damage; Cell-metabolism; Cellular-reactions; Diesel-emissions; Exposure-methods; Genes; Genetics; Genotoxic-effects; Genotoxicity; Health-hazards; Inhalation-studies; Mammalian-cells; Models; Mutagenesis; Organic-solvents; Particle-aerodynamics; Particulate-sampling-methods; Pulmonary-system-disorders; Particulate-dust; Risk-analysis; Respiratory-irritants; Solvents; Statistical-analysis; Toxic-effects
Contact
MJ Keane, Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Centers for Disease Control, 1095 Willowdale Road, Morgantown, WV 26505
CAS No.
1333-86-4
Publication Date
20090313
Document Type
Journal Article; Academic/Scholarly
Email Address
mjk3@cdc.gov
Fiscal Year
2009
NTIS Accession No.
NTIS Price
Issue of Publication
1
ISSN
1742-6596
NIOSH Division
HELD; PRL
Priority Area
Mining
Source Name
Journal of Physics: Conference Series, Inhaled Particles X, 23-25 September 2008, Sheffield, UK
State
WV
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