Development of an HPLC - MS procedure for the quantification of N-acetyl-S-(n-propyl)-L-cysteine, the major urinary metabolite of 1-bromopropane in human urine.
Cheever-KL; Marlow-KL; B'Hymer-C; Hanley-KW; Lynch-DW
J Chromatogr B 2009 Mar; 877(8-9):827-832
An analytical procedure was developed for the detection and quantification of N-acetyl-S-(npropyl)- l-cysteine (n-propylmercapturic acid, AcPrCys), a metabolite and biomarker for exposure to 1-bromopropane (1-BP). 1-BP is used as an industrial solvent and exposure is a health concern for industrial workers due to its toxicity. It has been associated with neurological disorders in both animals and humans. Urine sample preparation for the determination of AcPrCys consisted of solid phase extraction (SPE). Urine samples on preconditioned SPE (C18) columns were washed with 40% methanol/60% water solution prior to elution with acetone. Quantificationwas by means of a liquid chromatograph (LC) equipped with a mass spectrometer (MS) using an Aqua 3mu m C18 300A column and [d7]-AcPrCys was used as internal standard. Electrospray ionization (ESI)was used with the MS operated in the negative ion mode and selected ion monitoring (SIM) at m/z 204 for AcPrCys and m/z 211 for [d7]-AcPrCys. Demonstrated recovery of urine samples fortified at multiple levels (0.625-10mu g/ml) varied between 96 and 103% of theory with relative standard deviations (RSD) of 6.4% or less. The limit of detection (LOD) for the procedure was approximately 0.01mu g/ml AcPrCys in urine. These data will be discussed as well as other factors of the development of this test procedure.
Animals; Biochemistry; Biological-effects; Biological-factors; Biological-monitoring; Biological-transport; Biomarkers; Chemical-hypersensitivity; Chemical-properties; Chemical-reactions; Exposure-levels; Health-hazards; Humans; Industrial-equipment; Industrial-exposures; Industrial-hazards; Industrial-health-programs; Industrial-processes; Ion-exchange-chromatography; Ionization; Metabolic-study; Metabolites; Neurological-reactions; Neurophysiology; Neurotoxic-effects; Neurological-system; Neurological-diseases; Quantitative-analysis; Solvents; Toxic-effects; Toxicopathology; Toxins; Urinalysis; Work-areas; Work-environment; Worker-health; Workplace-studies;
Author Keywords: 1-Bromopropane; HPLC-MS
C. B'Hymer, U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, 4676 Columbia Parkway, Cincinnati, OH 45226,
Services; Healthcare and Social Assistance
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences