Skip directly to search Skip directly to A to Z list Skip directly to page options Skip directly to site content

NIOSHTIC-2 Publications Search

Search Results

Simultaneous measurement of mechanical responses and transepithelial potential difference and resistance, in guinea-pig isolated, perfused trachea using a novel apparatus: pharmacological characterization.

Authors
Jing-Y; Dowdy-JA; Van Scott-MR; Fedan-JS
Source
Eur J Pharmacol 2008 Nov; 598(1-3):98-103
NIOSHTIC No.
20034726
Abstract
The isolated, perfused trachea preparation has been used to compare reactivity of the intact airway in response to differential exposure of the mucosal (intraluminal) and serosal (extraluminal) surfaces to contractile and relaxant agonists and other agents, and to gain insight into the modulatory role of the epithelium and the pathways involved. The apparatus has also been configured for simultaneous measurement of transepithelial potential difference and changes in tracheal diameter, thereby providing parallel observations of epithelial and smooth muscle function and reactivity to drugs. The transepithelial potential difference is a product of transepithelial resistance and short circuit current, and the present study describes a novel isolated, perfused tracheal apparatus which allows simultaneous measurement of transepithelial potential difference, transepithelial resistance and mechanical responses of the smooth muscle. The apparatus was validated using well-known ion transport inhibitors [intraluminal amiloride and 5-nitro-2-(3-phenylpropyl-amino) benzoic acid (NPPB), extraluminal ouabain and bumetanide], bronchoactive agonists (extraluminal methacholine, histamine and terbutaline), and osmolytes (intraluminal D-mannitol and NaCl) to induce epithelium-derived relaxing factor-mediated relaxations. This apparatus will facilitate investigation of interactions between the epithelium and smooth muscle in airways that retain their in situ structure, and signaling mechanisms potentially involved in the regulation of airway smooth muscle tone.
Keywords
Airway-obstruction; Mucous-membranes; Muscle-relaxants; Reaction-rates; Cell-function; Cellular-function; Muscle-function; Muscle-physiology; Muscle-tissue
Contact
Jeffrey S. Fedan, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505
CODEN
EJPHAZ
Publication Date
20081119
Document Type
Journal Article
Email Address
jsf2@cdc.gov
Fiscal Year
2009
NTIS Accession No.
NTIS Price
Issue of Publication
1-3
ISSN
0014-2999
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
European Journal of Pharmacology
State
WV
TOP