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Determination of ortho-phthalaldehyde in air and on surfaces.

Authors
Tucker-SP
Source
J Environ Monit 2008 Nov; 10(11):1337-1349
NIOSHTIC No.
20034705
Abstract
Three sampling and analytical methods have been developed and evaluated for ortho-phthalaldehyde (OPA): (1) an HPLC-UV method for OPA in air, (2) a fluorimetric method for OPA on surfaces, and (3) a colorimetric method for OPA on surfaces. (1) The air sampler contains 350 mg of silica gel coated with 1 mg of acidified 2,4-dinitrophenylhydrazine (DNPH). Air sampling may be conducted at 0.03 to 1.0 L min-1 for periods up to 8 h. Samples were eluted with ethyl acetate, and the eluents were allowed to stand for 72 h. Analysis was by high performance liquid chromatography (HPLC) with a UV detector set at 369 nm. An unusual phenomenon was the observation that the stability of the sample on a sampler at 3 C tends to decrease as the total quantity of OPA collected on the sampler decreases. Elution of the samples within 24 h of air sampling is required. The detection limit (LOD) is approximately 0.02 g of OPA per sample. OPA on surfaces may be collected with strips cut from a sheet of polyvinyl alcohol (PVA wipe). (2) In the surface wipe method with analysis by fluorescence measurement, the strips of PVA wipe were placed into dimethyl sulfoxide. An aliquot was treated with aqueous N-acetyl-L-cysteine and ethylenediamine. Analysis was performed with a portable fluorometer (excitation and emission wavelengths = 365 nm and 438 nm, respectively). The LOD is 0.2 g per sample. (3) In the surface wipe method with visual colorimetric detection, the strips of PVA wipe were placed into 30 : 70 acetonitrile : water. An aliquot was treated with N-(1-naphthyl)ethylenediamine in 0.1 M sulfuric acid. After color development, the LOD is approximately 48 g per sample. These methods have been field tested in a hospital.
Keywords
Sampling; Analytical-chemistry; Analytical-processes; Analytical-methods
Contact
National Institute for Occupational Safety and Health, Cincinnati, OH 45226, USA
CODEN
JEMOFW
Publication Date
20081101
Document Type
Journal Article
Email Address
spt1@cdc.gov
Fiscal Year
2009
NTIS Accession No.
NTIS Price
Issue of Publication
11
ISSN
1464-0325
NIOSH Division
DART
Priority Area
Healthcare and Social Assistance
Source Name
Journal of Environmental Monitoring
State
OH
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