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Vibration causes acute vascular injury in a two-step process: vasoconstriction and vacuole disruption.

Authors
Govindaraju-SR; Bain-JL; Eddinger-TJ; Riley-DA
Source
Anat Rec 2008 Aug; 291(8):999-1006
NIOSHTIC No.
20034583
Abstract
Hand-arm vibration syndrome is a vasospastic and neurodegenerative occupational disease. In the current study, the mechanism of vibration-induced vascular smooth muscle cell (SMC) injury was examined in a rat-tail vibration model. Tails of male Sprague Dawley rats were vibrated continuously for 4 hr at 60 Hz, 49 m/s(2) with or without general anesthesia. Ventral tail arteries were aldehyde fixed and embedded in epoxy resin to enable morphological analysis. Vibration without anesthesia caused vasoconstriction and vacuoles in the SMC. Anesthetizing rats during vibration prevented vasoconstriction and vacuole formation. Exposing tail arteries in situ to 1 mM norepinephrine (NE) for 15 min induced the greatest vasoconstriction and vacuolation. NE induced vacuoles were twice as large as those formed during vibration. When vibrated 4 hr under anesthesia after pretreatment with NE for 15 min, the SMC lacked vacuoles and exhibited a longitudinal banding pattern of dark and light staining. The extracellular matrix was filled with particulates, which were confirmed by electron microscopy to be cellular debris. The present findings demonstrate that vibration-induced vasoconstriction (SMC contraction) requires functioning central nervous system reflexes, and the physical stress of vibration damages the contracted SMC by dislodging and fragmenting SMC vacuoles.
Keywords
Vibration; Vibration-disease; Vibration-control; Vibration-effects; Vibration-exposure; Neurovascular-disorders; Animal-studies; Animals; Laboratory-animals; Biological-effects; Biological-monitoring; Musculoskeletal-system-disorders; Musculoskeletal-system; Author Keywords: hand-arm vibration; rat-tail artery; smooth muscle cells; norepinephrine
Contact
Danny A. Riley, Department of Cell Biology, Neurobiology & Anatomy, 8701 Watertown Plank Road, Medical College of Wisconsin, Milwaukee, WI 53226
CODEN
ARNEGY
Publication Date
20080801
Document Type
Journal Article
Email Address
dariley@mcw.edu
Funding Amount
922402
Funding Type
Grant
Fiscal Year
2008
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-003493
Issue of Publication
8
ISSN
1932-8486
Priority Area
Manufacturing; Wholesale and Retail Trade
Source Name
Anatomical Record
State
WI
Performing Organization
Medical College of Wisconsin, Milwaukee, Wisconsin
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