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In vitro biologic activities of the antimicrobials triclocarban, its analogs, and triclosan in bioassay screens: receptor-based bioassay screens.

Authors
Ahn-KC; Zhao-B; Chen-J; Cherednichenko-G; Sanmarti-E; Denison-MS; Lasley-B; Pessah-IN; Kültz-D; Chang-DP; Gee-SJ; Hammock-BD
Source
Environ Health Perspect 2008 Sep; 116(9):1203-1210
NIOSHTIC No.
20034577
Abstract
BACKGROUND: Concerns have been raised about the biological and toxicologic effects of the antimicrobials triclocarban (TCC) and triclosan (TCS) in personal care products. Few studies have evaluated their biological activities in mammalian cells to assess their potential for adverse effects. OBJECTIVES: In this study, we assessed the activity of TCC, its analogs, and TCS in in vitro nuclear-receptor-responsive and calcium signaling bioassays. MATERIALS AND METHODS: We determined the biological activities of the compounds in in vitro, cell-based, and nuclear-receptor-responsive bioassays for receptors for aryl hydrocarbon (AhR), estrogen (ER), androgen (AR), and ryanodine (RyR1). RESULTS: Some carbanilide compounds, including TCC (1-10 muM), enhanced estradiol (E(2))-dependent or testosterone-dependent activation of ER- and AR-responsive gene expression up to 2.5-fold but exhibited little or no agonistic activity alone. Some carbanilides and TCS exhibited weak agonistic and/or antagonistic activity in the AhR-responsive bioassay. TCS exhibited antagonistic activity in both ER- and AR-responsive bioassays. TCS (0.1-10 muM) significantly enhanced the binding of [(3)H]ryanodine to RyR1 and caused elevation of resting cytosolic [Ca(2+)] in primary skeletal myotubes, but carbanilides had no effect. CONCLUSIONS: Carbanilides, including TCC, enhanced hormone-dependent induction of ER- and AR-dependent gene expression but had little agonist activity, suggesting a new mechanism of action of endocrine-disrupting compounds. TCS, structurally similar to noncoplanar ortho-substituted poly-chlorinated biphenyls, exhibited weak AhR activity but interacted with RyR1 and stimulated Ca(2+) mobilization. These observations have potential implications for human and animal health. Further investigations are needed into the biological and toxicologic effects of TCC, its analogs, and TCS.
Keywords
Biological-effects; Biological-monitoring; Microbiology; Microorganisms; Microscopic-analysis; Bacteria; Bacterial-disease; Bioassays; Genetic-factors; Genotoxic-effects; Hormone-activity; Toxic-effects; Toxicology
Contact
Bruce D. Hammock, Department of Entomology, University of California, Davis, Davis, CA 95616
CODEN
EVHPAZ
Publication Date
20080901
Document Type
Journal Article
Email Address
bdhammock@ucdavis.edu
Funding Type
Agriculture; Cooperative Agreement
Fiscal Year
2008
NTIS Accession No.
NTIS Price
Identifying No.
Cooperative-Agreement-Number-U50-OH-007550
Issue of Publication
9
ISSN
0091-6765
Source Name
Environmental Health Perspectives
State
CA
Performing Organization
University of California - Davis
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