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Increased accumulation of neutrophils and decreased fibrosis in the lung of NADPH oxidase-deficient C57BL/6 mice exposed to carbon nanotubes.

Authors
Shvedova-AA; Kisin-ER; Murray-AR; Kommineni-C; Castranova-V; Fadeel-B; Kagan-VE
Source
Toxicol Appl Pharmacol 2008 Sep; 231(2):235-240
NIOSHTIC No.
20034404
Abstract
Single-walled carbon nanotubes (SWCNT) have been introduced into a large number of new technologies and consumer products. The combination of their exceptional features with very broad applications raised concerns regarding their potential health effects. The prime target for SWCNT toxicity is believed to be the lung where exposure may occur through inhalation, particularly in occupational settings. Our previous work has demonstrated that SWCNT cause robust inflammatory responses in rodents with very early termination of the acute phase and rapid onset of chronic fibrosis. Timely elimination of polymorphonuclear neutrophils (PMNs) through apoptosis and their subsequent clearance by macrophages is a necessary stage in the resolution of pulmonary inflammation whereby NADPH oxidase contributes to control of apoptotic cell death and clearance of PMNs. Thus, we hypothesized that NADPH oxidase may be an important regulator of the transition from the acute inflammation to the chronic fibrotic stage in response to SWCNT. To experimentally address the hypothesis, we employed NADPH oxidase-deficient mice which lack the gp91phox subunit of the enzymatic complex. We found that NADPH oxidase null mice responded to SWCNT exposure with a marked accumulation of PMNs and elevated levels of apoptotic cells in the lungs, production of pro-inflammatory cytokines, decreased production of the anti-inflammatory and pro-fibrotic cytokine, TGF-, and significantly lower levels of collagen deposition, as compared to C57BL/6 control mice. These results demonstrate a role for NADPH oxidase-derived reactive oxygen species in determining course of pulmonary response to SWCNT.
Keywords
Toxins; Particulate-dust; Dust-inhalation; Dust-exposure; Pulmonary-congestion; Pulmonary-function; Pulmonary-system; Pulmonary-system-disorders; Lung-disorders; Lung-irritants; Laboratory-animals; Laboratory-testing; Aerosol-particles; Cell-biology; Cell-function; Cellular-function; Cellular-respiration; Oxidation; Oxidative-metabolism; Oxidative-processes; Nanotechnology
Contact
A.A. Shvedova, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Pathology and Physiology Research Branch, Health Effects Laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505
CODEN
TXAPA9
Publication Date
20080901
Document Type
Journal Article
Email Address
ats1@cdc.gov
Funding Type
Grant
Fiscal Year
2008
NTIS Accession No.
NTIS Price
Identifying No.
Grant-Number-R01-OH-008282
Issue of Publication
2
ISSN
0041-008X
NIOSH Division
HELD
Priority Area
Manufacturing
Source Name
Toxicology and Applied Pharmacology
State
WV; PA
Performing Organization
University of Pittsburgh at Pittsburgh
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