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Unrestrained acoustic plethysmograph for measuring specific airway resistance in mice.

Authors
Reynolds-JS; Johnson-VJ; Frazer-DG
Source
J Appl Physiol 2008 Aug; 105(2):711-717
NIOSHTIC No.
20034350
Abstract
An acoustic whole body plethysmograph was developed to estimate specific airway resistance (sRaw) in unrestrained mice. The plethysmograph uses acoustic principles to measure the thoracic breathing pattern and simultaneously measures the airflow entering and/or leaving the plethysmograph. Similarly to traditional methods utilizing a double-chamber plethysmograph, these measurements were combined to estimate sRaw. To evaluate the new system, we placed six conscious A/J mice individually in a whole body plethysmograph (Buxco System) for a 2-min exposure to aerosolized methacholine chloride dissolved in saline (0, 5, 10, and 20 mg/ml), which is known to increase sRaw in mice. Three minutes after exposure, the mice were transferred to the acoustic plethysmograph for 2 min for data collection. The mean baseline value of sRaw was 0.93+/-0.10 cmH2O.s. A dose-dependent increase in sRaw was shown, with an approximate tripling of sRaw at the highest dose. These results demonstrate the ability of the system to estimate sRaw based on plethysmograph airflow and acoustic amplitude.
Keywords
Laboratory-animals; Animals; Animal-studies; Plethysmography; Plethysmographs; Air-flow; Breathing; Lung-burden; Lung-function; Statistical-analysis; Acoustic-absorption; Acoustical-measurements; Acoustic-trauma
Contact
J. S. Reynolds, Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road - ms L2101, Morgantown, WV 26505, USA
CODEN
JAPHEV
Publication Date
20080801
Document Type
Journal Article
Email Address
jsr0@cdc.gov
Fiscal Year
2008
NTIS Accession No.
NTIS Price
Issue of Publication
2
ISSN
8750-7587
NIOSH Division
HELD
Priority Area
Construction; Manufacturing
Source Name
Journal of Applied Physiology
State
WV
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